Common carps (Cyprinus carpio) with average body weight of (10±1.2) g were randomly divided into five treatments with three replicates (35 fish in each treatment),to investigate the effect of dietary protein level on hepatic function and antioxidant capacity by feeding diets at five protein levels (30%,30.5%,31%,31.5% and 32%). Every four weeks,the related physiological and biochemical indices were determined. At the 4th and 8th week,the results show:1) Activities of alanine transaminase (ALT) and aspartate transaminase (AST) in serum and hepatopancreas in 31% protein group were significantly higher (P<0.05). 2) Compared with the fish fed with the other treatment groups,those fed with 31% protein group had significantly higher activities of superoxide dismutase (SOD),catalase (CAT) and lysozyme (LZM) (P<0.05). 3) Contents of malonaldehyde (MDA) of fish which were fed with 31% protein group decreased significantly (P<0.05). Considering the cost of feed preparation for common carps,dietary protein of 31% meets the requirement for hepatic function and antioxidant capacity for the juveniles.%研究了不同蛋白水平饲料对鲤(Cyprinus carpio)幼鱼肝功能和抗氧化能力的影响.选用体质量为(10±1.2)g的健康鲤幼鱼,随机分为5组,每组设3个重复,每个重复35尾鱼.放养在15个规格相同的白色水箱中,分别投喂蛋白水平为30%、30.5%、31%、31.5%和32%的饲料(分别记作Diet 1、Diet 2、Diet 3、Diet 4和Diet 5),饲养实验时间为8周,每隔4周对相关生理生化指标进行测定.结果表明,养殖第4和第8周时,31%蛋白水平组血清和肝胰脏的谷丙转氨酶(ALT)和谷草转氨酶(AST)活性均显著降低(P<0.05);血清、肝胰脏和肾脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和溶菌酶(LZM)活性升高(P<0.05),丙二醛(MDA)浓度显著降低(P<0.05).根据实验结果,从饲料配制的成本考虑,31%蛋白水平饲料能够达到维持鲤幼鱼机体正常的抗氧化能力以及保护肝功能的营养需求.
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