高效液相色谱法同时测定土壤中环丙氨嗪和三聚氰胺

摘要

本试验研究建立了同时测定土壤中环丙氨嗪和三聚氰胺残留量的高效液相色谱法.红壤、潮土等5种土壤样品经氨水/甲醇 (5/95,v/v)超声提取3次,浓缩处理后上机检测.环丙氨嗪和三聚氰胺的标准曲线在0.1 ～ 15.0 μg/ml浓度范围内线性关系良好,绝对系数(R~2)分别为1.0000和0.9998;在0.5 ～ 5 mg/kg添加范围内,环丙氨嗪和三聚氰胺在土壤中的平均回收率分别为87.2% ～ 101.1% 和 75.3% ～ 101.6%,变异系数分别为3.3% ～ 8.1%、1.6% ～ 9.9%,最低检测限分别为0.05 mg/kg、0.07 mg/kg.与国际上气相/液相色谱-质谱连用法相比,操作简单,经济方便易于普及.%An analytic method with HPLC was developed to determine the residues of cyromazine and melamine in soils. Soil samples were extracted thrice with ammonia/methanol (5/95,v/v) by ultrasonic and concentrated, and then analyzed by HPLC. The calibration curves for cyromazine and melamine were linear in concentration range of 0.1-15.0 μg/ml (R~2=1.0000 and 0.9998, respectively) . In the concentration of 0.5-5 mg/kg, the average recoveries of cyromazine and melamine were 87.2% - 101.1% and 75.3% - 101.6% with the variation coefficient of 3.3% - 8.1% and 1.6% - 9.9% respectively. The limit of detection was 0.05 mg/kg and 0.07 mg/kg, respectively.