青蒿琥酯对乳腺癌细胞株MDA-MB-231生长的影响

摘要

Obective To study the. inflence of artesunate on proliferation of breast cancer MDA-MB-231 cells and it's possible mechanism. Methods Use different concentrations( 0、25、50 、1OOμg/ml) of Artesunate to treat the MDA-MB-231 cells. The MTT assay was used to detect the growth of cells ; the cell proliferation index was measured by microscope and the cell cycle( mainly the ratio of G2/M phase and apoptosis cells) was analyed by flow cytometry. Results The results of MTT assay showed Artesunate could inhibit the proliferation of MDA-MB-231 cells , with the increase of drug concentration and time treated by drugs, the inhibitory action also raised. When compared the experimental groups with the control grop , there are sighificant differences analysed by the statistical software( P ＜ 0. 05 ) . The ratio of cells in G2 phase and/or mitotic phase and the proportion of apoptotic cells increased significantly after treated by Artesunate analyzed by the results of cell proliferation index and the flow cytometry. when compared with the control group, the difference has statistical meaning( P ＜ 0. 05) . Conclusion Artesunate can inhibit the proliferation of MDA-MB-231 cells by prohibiting their division and induce apoptosis, Artesunate can be used as a potential drug to treat hreast cancer.%目的 探讨青蒿琥蒿酯对乳腺癌细胞株MDA-MB-231增殖的影响及可能机制.方法 以不同浓度(0、25、50、100μg/ml)的青蒿琥酯处理MDA-MB-231细胞,然后用MTT(四甲基偶氮唑盐)法检测各组细胞生长情况,用显微镜计数各组分裂期细胞所占比例,用流式细胞仪分析细胞周期(主要为C2/M期细胞和凋亡细胞比例).结果 MTT显示青蒿琥酯对MDA-MB-231细胞增殖有抑制作用,且随着药物浓度的增加和药物处理时间的延长,抑制作用增加,各实验组与对照组比较,经统计分析差异有统计学意义(P＜0.05);细胞分裂指数结果、流式细胞仪分析结果显示青蒿琥酯可明显增加MDA-MB-231细胞的分裂期细胞或G2/M比例和凋亡细胞比例,与时照组相比差异有统计学意义(P＜0.05).结论 青蒿琥酯可通过抑制MDA-MB-231细胞分裂而阻碍其增殖,同时还具有诱导细胞凋亡的作用,青蒿琥酯可作为治疗乳腺的一个潜在药物.