目的 探讨微囊化异种嗅球组织移植联合L-NAME对脊髓继发性损伤的治疗保护作用及机制.方法 健康SD大鼠,随机分为对照组、微囊化兔嗅球组织移植组、微囊化异种嗅球组织移植联合LNAME治疗组,损伤组又设1、3、7、14、28d 5个时相点,紫外分光光度计检测脊髓伊文氏蓝含量,干湿重法测定脊髓组织含水量;免疫组织化学技术检测MMP-9的表达;神经功能评分法(BBB法)评价大鼠术后运动功能的恢复情况.结果 脊髓损伤后脊髓伊文氏蓝含量增加、脊髓血管源性水肿、MMP-9表达上调,大鼠的运动功能缺失,经微囊化异种嗅球组织移植联合L-NAME治疗后血脊髓屏障通透性降低、水肿明显减轻、MMP-9表达下调,大鼠的运动功能恢复.结论 微囊化异种嗅球组织移植对脊髓继发性损伤有较好的疗效,联合应用嗅球组织细胞和L-NAME在脊髓损伤修复治疗中具有协同作用.%Objective To explore the protective effects and mechanism of exnotransplantation of microencapsulated olfactory bulb combined with L-NAME in rats at the early stages of spinal cord injury. Methods Healthy SD rats were divided into three groups as following: injury control group, microencapsulated transplantation of rabbit olfactory bulb cells group, microencapsulated transplantation of rabbit olfactory bulb cells combined with L-NAME group, the later two groups were quantified at 1, 3,7, 14, and 28 d post spinal cord injury. Spinal Evans blue content was examined with the ultraviolet spectrophotometer; Spinal cord water content was detected by caculating dry/wet weight. The change of MMP-9 expression after spinal cord injury was determined by immunohistochemical method. BBB score was designed to assess its behavioral ability. Results Evans blue content and expression of MMP-9 were significantly were significantly increased, vasogenic spinal cord edema, and loss of motor function in rats after spinal cord irjury. Permeability of blood-spinal barrier and vasogenic spinal cord edema, MMP-9 were decreased, motor function was recoveried in rats after they were treated with microencapsulated transplantation of rabbit olfactory bulb cells combined with L-NAME. Conclusion Exnotransplantation of microencapsulated strategy of olfactory ensheating cells was effective in the repair of spinal cord injury in some extent. There may be a synergistic effect between olfactory ensheating cells and L-NAME.
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