一种新型蚯蚓急性毒性测试方法的探索

摘要

Withλ-Cyhalothrin as the experimental reagent,Da ping Ⅱ earthworms as the selected objects,this study poisoned earthworms by adding different λ-Cyhalothrin concentrations to the dung of earthworms to analyze the probability of poisoned earthworms by using the statistical software named SPSS19.0.Then calculate the LC50 ofλ-Cyhalothrin to earthworms.Results show that the LC50 of λ-Cyhalothrin to earthworms is about 481 mg/kg,belongs to low toxicity pesticides,which is consistent with the result of the traditional experimental method.%以高效氯氟氰菊酯为实验试剂,大平二号蚯蚓为供试对象,采用向蚓粪中施用不同浓度药剂的方法使蚯蚓染毒,利用SPSS19.0统计软件对蚯蚓的中毒条数进行概率回归分析,继而计算出高效氯氟氰菊酯对蚯蚓的半数致死浓度LC50.结果表明:高效氯氟氰菊酯对蚯蚓的半数致死浓度LC50约为481 mg/kg,属于低毒农药,与传统实验方法得出的实验结果一致.