电针对糖尿病胃轻瘫大鼠胃动素及生长抑素的影响

摘要

目的：观察电针对糖尿病胃轻瘫(DGP)大鼠胃动素(MOT)及生长抑素(SS)的影响。方法选取尿糖阴性清洁级健康SD大鼠100只,稳定饲养3 d后,随机选取30只为对照组,其余70只采用链脲佐菌素造模建立胰岛素依赖型糖尿病动物模型,10星期后随机抽取造模成功的大鼠分为模型组和治疗组,每组30只,模型组不做任何治疗,电针组取足三里、中脘、胃俞予电针治疗。观察各组治疗前后血清及胃窦组织MOT和SS变化情况。结果治疗组和模型组大鼠体重及胃肠推进率与对照组比较,差异均具有统计学意义(P＜0.01)。治疗组治疗后体重及胃肠推进率与模型组比较,差异均具有统计学意义(P＜0.01)。治疗组和模型组大鼠造模后、治疗10星期后及治疗后血糖值与对照组比较,差异均具有统计学意义(P＜0.01)。治疗组治疗后血糖值与同组治疗10星期后比较,差异具有统计学意义(P＜0.01)。治疗组和模型组血清及胃窦组织中MOT和SS含量与对照组比较,差异均具有统计学意义(P＜0.01)。治疗组治疗后血清及胃窦组织中MOT和SS含量与模型组比较,差异均具有统计学意义(P＜0.01)。结论电针可使DGP大鼠血清中SS及胃窦组织中MOT显著上升,血清中MOT及胃窦组织中SS相应下降,从而改善DGP大鼠血中高胃动素及高生长抑素状态。%Objective To investigate the effect of electroacupuncture on motilin (MOT) and somatostatin (SS) in rats with diabetes gastroparesis (DGP). Methods One hundred clean healthy SD rats with negative urine glucose were chosen. After they were fed for three days and into a stable state, 30 rats were randomly selected as a control group. The other 70 rats were used as an animal model of insulin-dependent diabetes made with streptozotocin. At 10 weeks after model making, rats as successful models were randomly allocated to model and treatment groups, 30 rats each. The model group received no treatment and the treatment group, electroacupuncture at points Zusanli, Zhongwan and Weishu. Serum and antral MOT and SS were measured in every group before and after treatment. Results There were statistically significant differences in body weight and gastrointestinal propulsion rate between the treatment or model group of rats and the control group (P<0.01), and between the treatment and model groups after treatment (P<0.01). There was a statistically significant difference in blood glucose value between the treatment or model group of rats and the control group after model making and at 10 weeks and the end of treatment (P<0.01). There was a statistically significant difference in blood glucose value between at the end and 10 weeks of treatment in the treatment group (P<0.01). There were statistically significant differences in serum and antral MOT and SS contents between the treatment or model group and the control group (P<0.01), and between the treatment and model groups after treatment (P<0.01). Conclusions Electrocupuncture can markedly increase serum SS and antral MOT and decrease serum MOT and antral SS to ameliorate high blood motilin and somatostatin in DGP rats.