温针灸对吗啡戒断大鼠学习记忆能力及前额叶皮质区 CaMKⅡ含量的影响

摘要

Objective To investigate the effect of warm needling on learning and memory abilities and the calmodulin kinaseⅡ (CaMKⅡ) content of prefrontal cortex area in morphine withdrawal rats andexplore the mechanism of its action. Methods Forty clean-grade male SD rats were randomly allocated to control, model, manual needling and warm needling groups, 10rats each. A SD rat model of morphine addiction and withdrawal was made by dorsal subcutaneous injection of day-by-day incremental morphine and rapid withdrawal with Naloxone after addiction. Learning and memory abilities were tested using a Morris water maze and the CaMKⅡ content of prefrontal cortex area was measured by an immunohistochemical method in every group of rats.Results There were statistically significant differences in mean platform escape latency, the number of platform crossing and the CaMKⅡ content of PFC area between the control, manual needling or warm needling group of rats and the model group (P<0.01). There were statistically significant differences in mean platform escape latency, the number of platform crossing and the CaMKⅡ content of PFC area between the warm needling and manual needling groups (P<0.05).Conclusions Warm needling treatment can restore learning and memory abilities in morphine withdrawal rats. The mechanism of its action may be related to an increase in the CaMKⅡ content of prefrontal cortex area.%目的：观察温针灸对吗啡成瘾戒断后大鼠学习记忆能力及前额叶皮质区钙调蛋白激酶Ⅱ(CaMKⅡ)含量的影响，并进一步探讨其作用机制。方法将40只清洁级雄性SD大鼠随机分为对照组、模型组、手针组和温针组，每组10只。模型组、手针组和温针组大鼠背部皮下注射吗啡，逐日递增吗啡的注射剂量，成瘾后给予纳洛酮快速戒断，建立吗啡成瘾戒断后 SD 大鼠模型。采用水迷宫试验测定各组大鼠的学习记忆能力，并采用免疫组化法测定各组大鼠前额叶皮质区 CaMKⅡ含量。结果对照组、手针组及温针组大鼠平台逃避平均潜伏期、穿越平台次数及PFC区CaMKⅡ含量与模型组比较，差异均具有统计学意义(P＜0.01)。温针组大鼠平台逃避平均潜伏期、穿越平台次数及PFC区CaMKⅡ含量与手针组比较，差异均具有统计学意义(P＜0.05)。结论温针灸治疗可以恢复吗啡戒断后大鼠的学习记忆能力，其作用机制可能与吗啡戒断后前额叶皮质区CaMKⅡ含量增加有关。