慢性阻塞性肺疾病急性加重期血清MMP-9、TIMP-1变化的临床意义

摘要

Objective To investigate the MMP-9, TIMP-1 serum level in patients with AECOPD and their clinical significance. Methods Fifty-five AECOPD patients (COPD group) and twenty healthy controls (control group) were re-cruiied. According to lung function and severity, the AECOPD patients were divided into 3 groups; Mild group ( 15 cases) , Moderate group (22 cases) , Severe group (18 cases). Serum levels of MMP-9, TIMP-1 were measured by EUSA. Lung function and FEV/FVC, FEV,% were tested. Then the correlations between serum levels of MMP-9, TIMP-1 and severity were evaluated. Results The serum levels of MMP-9, TIMP-1 in COPD group were higher than those of control group, and they gradually increased with the severity of COPD (P <0. 01 or <0. 05). Linear correlation analysis showed that the levels of MMP-9, TIMP-1 negatively correlated with FEV,% (r= -0.783 or -0.776, all P<0.01). Conclusions The serum levels of MMP-9 and TIMP-1 gradually increase with the severity of COPD, and negatively correlated with FEV, %. It indicates that the changes of serum MMP-9 and TIMP-1 may be determined as an indicator of the severity of COPD.%目的 探讨慢性阻塞性肺疾病(COPD)急性加重期血清基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶组织抑制剂-1(TIMP-1)变化的临床意义.方法 选取COPD急性加重期患者55例(COPD组)、查体健康者20例(对照组),根据COPD组肺功能及病情程度分为轻度组l5例、中度组22例、重度组18例.采用酶联免疫吸附法检测各组血清MMP-9及TIMP-1；行肺功能通气检查,记录第1秒钟用力呼吸容积(FEV1)/用力肺活量(FVC)及FEV,占预计值的百分比(FEV1％).分析COPD组血清MMP-9、TIMP-1水平与病情严重程度的相关性.结果 COPD组血清MMP-9、TIMP-1明显高于对照组,且随病情加重逐渐升高(P＜0.01或＜0.05)；直线相关分析显示,COPD组血清MMP-9、TIMP-1水平与FEV1％呈负相关(r分别为-0.783、-0.776,p均＜0.01).结论 血清MMP-9、TIMP-1水平与COPD病情程度相关,病情越重,MMP-9、TIMP-1越高,且与FEV1％呈负相关；提示血清MMP-9、TIMP-1变化可作为判定COPD病情严重程度的指标之一.