沙眼衣原体临床株基因分型及体外药敏试验结果分析

摘要

Objective To assess the genotyping distribution of Chlamydia trachomatis( Ct) clinical isolates and its susceptibility to common antimicrobial agents; to improve the treatment level of urogenitaltract infection. Methods Poly-merase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was performed to determine the genotype of 52 Ct; the MICs of 9 antimicrobial agents to Ct were determined. Results There were 49 strains of E type, 2 strains of G type, and 1 strain of J type. The highest MIC of 9 antimicrobial agents to Ct was erythromycin, and the lowest was clar-ithromycin; 13 strains were found resistant. Conclusions The most prevalent genotypes of Ct infection in Tianjin is E type, which is susceptive to clarithromycin; the clinic should take attention to that Ct strains resistant to erythromycin have been found.%目的 分析沙眼衣原体(Ct)临床株的基因型分布及其对常用抗菌药物的敏感性:进一步提高泌尿生殖道Ct感染的治疗水平.方法 采用聚合酶链反应和限制性内切酶片段长度多态性分析方法对52株Ct行基因分型:测定9种抗菌药物对其的最低抑菌浓度(MIC).结果 52株Ct中E型49株、G型2株、J型1株.9种抗菌药物对Ct的MIC以红霉素最高,克拉霉素最低:共发现13株红霉素耐药株,未发现对其他药物的耐药株.结论 天津地区Ct感染以E型为主,对克拉霉素较为敏感:已发现对红霉素耐药的Ct,应引起临床注意.