TIMP-1在哈萨克族食管癌组织中的表达及其意义

摘要

目的 探讨基质金属蛋白酶组织抑制物-1(TIMP-1)在哈萨克族食管癌组织中的表达及其与临床病理特征的关系.方法 采用RT-PCR检测48例哈萨克族食管癌切除标本与远端正常组织中TIMP-1 mRNA表达水平,并分析其与临床病理特征的关系;采用Western blot对20例标本中TIMP-1蛋白表达进行检测.结果 癌组织中TIMP-1 mRNA表达远高于其远端正常组织(P＜0.05);在临床早期病例中TIMP-1基因在癌组织及远癌正常组织中的差异亦具有统计学意义(P＜0.05),蛋白表达与基因转录表达水平基本一致.TIMP-1 mRNA表达在癌组织浸润深度、分化程度及有无淋巴结转移间差异无统计学意义.结论 TIMP-1可能参与了哈萨克族食管癌发生的早期过程.%Objective To study the expressions of TIMP-1 in Hazak's esophageal cancer tissues and investigate the relationship between their expressions and the clinical pathological features. Methods The mRNA expression levels of TIMP-1 in 48 Hazak's esophageal cancer specimens were investigated by RT-PCR. The relationship between clinical pathologic features and expressions of TIMP-1 mRNA level was analyzed. Western blot was used to detect the expressions of TIMP-1 protein in 20 samples. Results The expression of TIMP-1 mRNA in cancer tissues was significantly higher than that of normal tissues ( P ＜0.05), and there were significant differences about the expressions of TIMP-1 in the clinical early stage specimens(P＜0.05). The expressions of TIMP-1 protein and mRNA were identical basically. There were no significances of TIMP-1 expressions in differentiated degree, invasive depth and lymph node metastasis. Conclusions The expressions of TIMP-1 may play an important role in early stage of Hazak' s esophageal cancer.