不同时间低氧条件对人胃黏膜上皮细胞凋亡的影响

摘要

目的 研究低氧条件对体外培养人胃黏膜上皮细胞株GES-1凋亡的影响.方法 建立GES-1细胞体外低氧培养模型.在低氧处理后不同时间点(24 h 、48 h、72 h)MTT法检测存活率、电镜观察形态变化、TUNEL染色观察细胞凋亡.结果 低氧条件作用24 h、48 h、72 h后,GES-1细胞存活率均下降(P＜0.05),细胞凋亡指数均升高(P＜0.05),透射电镜均观察到部分凋亡细胞.结论 低氧条件可明显抑制GES-1细胞生长并诱导其凋亡.%Objective To investigate the effect of hypoxia on apoptosis in human gastric epithelial cell strain-1 ( GES-1). Methods Hypoxia culture model in vitro was established to test the apoptosis in GES-1 cell line. After GES-1 was cultured in hypoxia circumstance for a given period of time (24 h, 48 h, 72 h). The survival rate of cell and apoptosis were measured by MTT, electromicroscope, TUNEL staining. Results The survival rate of GES-1 was decreased (P < 0.05) , but the apoptosis index was increased (P<0. 05) in hypoxia at 24 h, 48 h, 72 h. The transmission electron microscope showed some apoptosis-like morphologic changes. Conclusion Hypoxia has an obvious inhibitory effect on the cell proliferation, and increases apoptosis of GES-1.