鼻咽癌中PTTG1与MAPK/ERK信号通路及增殖凋亡的关系

摘要

目的 探讨鼻咽癌中PTTG1与MAPK/ERK信号通路及细胞增殖凋亡的关系.方法 采用免疫组化SP法检测33例鼻咽癌中PTTG1、p-ERK、Ki-67蛋白的表达；用50、100 μmol/L的ERK/MAPK信号传导通路抑制剂PD98059,分别处理CNE-2Z细胞24、48、72 h,免疫细胞化学法检测PTTG1、p-ERK、Ki-67蛋白的表达；MTT法检测细胞增殖抑制率；流式细胞仪检测细胞凋亡.结果 33例鼻咽癌组织中PTTG1、p-ERK、Ki-67蛋白的阳性表达率分别为81.82％ (27/33)、66.67％ (22/33)、78.79％ (26/33)；PTTG1与p-ERK(r=0.345,P=0.042 ＜0.05)、Ki-67(r =0.600,P＜0.01)蛋白表达均呈正相关；不同浓度PD98059作用于CNE-2Z细胞不同的时间,免疫细化检测PT-TG1、p-ERK、Ki-67蛋白的阳性表达降低,细胞增殖明显受抑制,可检测到凋亡峰.结论 PTTG1在鼻咽癌中可能通过MAPK/ERK信号通路发挥促进癌细胞增殖及抑制凋亡的生物学作用.%Objective To investigate the relationship between PTTG1 and MAPK/ERK signalpathway, proliferation, apoptosis in nasopharyngeal carcinoma ( NPC). Methods Immunohistochemistry SP method was adopted to detect the expression of PTTG1, p-ERK, Ki-67 protein in 33 cases NPC. CNE-2Z cells was treated with different dose of ERK/MAPK signal way inhibitors PD98059 (50 μmol/L, 100 μmol/L) , and immunohistochemistry SP method was adopted to detect the expression of PTTG1, p-ERK, Ki-67 protein, MTT was adopted to detected proliferation, flow cytometry was used to observe apoptosis. Results Positive staining rates of PTTG1, p-ERK, Ki-67 protein in 33 cases NPC was 81. 82% (27/ 33) , 66.67% (22/33), 78. 79% (26/33) , respectively. There was statistically significant positive correlations between positive expression of PTTG1 and p-ERK (r = 0. 345, P < 0.05) , Ki-67 (r =0. 600, P < 0. 01) protein. When CNE-2Z cells treated by different time and different dose of PD98059, the expression of PTl'Gl, p-ERK, Ki-67 protein was decreased, CNE-2Z cellular proliferation was restrained remarkably, apoptosis can be observed. Conclusion PTTG1 may play biology effect through MAPK/ERK signal pathway promoting cellular proliferation and inhibiting apoptosis in NPC.