二十味沉香丸对低压低氧暴露大鼠血红蛋白、红细胞比容及血清EPO表达的影响

摘要

目的 探讨二十味沉香丸对低压低氧暴露大鼠血红蛋白(Hb)、红细胞比容(Hct)及血清促红细胞生成素(EPO)表达的影响.方法 将165只Wistar大鼠随机分为平原对照组(15只)、高原对照组(75只)和高原给药组(75只).平原对照组即刻检测Hb、Hct,采集血清并液氮储存;将高原对照组和高原给药组大鼠暴露于低压低氧环境.高原对照组用0 9％生理盐水灌胃,高原给药组用二十味沉香丸制成的水剂灌胃.高原对照组、高原给药组分别于1、3、7、15、30 d,各取15只大鼠,测定大鼠体质量、Hb、Hct,用ELISA法检测血清EPO.结果 高原对照组大鼠高原暴露第3、7、15、30天Hb和Hct水平较平原对照组有所升高,但明显低于高原给药组(P均＜0.05).高原对照组血清EPO表达随大鼠暴露时间延长而增强(P均＜0.05).高原给药组血清EPO表达在大鼠暴露15 d左右略有升高(P＜0 05),但30 d时又恢复至正常水平.高原对照组血清EPO表达水平与Hb呈正相关(r=0.83,P＜0.05).结论 二十味沉香丸可降低低压低氧暴露大鼠的Hb和Hct,可能是通过干预EPO的表达起作用.%Objective To investigate the influences of Ershiwei Chenxiang Wan on Hb, Hct, and serum EPO of rats exposed to hypobaric hypoxia. Methods 165 Wistar rats were randomly divided into the control group in plains (n = 15) , the control group in plateau (n = 75 ) and the administrated group in plateau (n = 75 ). Hb and Hct had been immediatedly analyzed in the control group in plains and the serum of rats were collected and stored in the liquid nitrogen. The control group and the administrated group in plateau had been exposed under hypobaric hypoxia, and were treated with 0.9% normal saline, Ershiwei Chenxiang Wan, respectively. On 1, 3, 7, 15, and 30 day, Hb, Hct concentration and the rat body weight from each of 15 rats in the control group and the administrated group in plateau had been detected, and serum EPO were analyzed by ELISA. Results Hb and Hct concentration were enhanced in the control group in plateau than the control group in plains at 3 , 7, 15 , and 30 days explosion (all P <0.05) , but it was significantly lower than the administrated group in plateau (all P<0.05). Expression of EPO was increased with the exposed time in the control group in plateau (all P<0. 05), and which was little enhanced after 15 days, but after 30 days explosion, recovered to normal level. Expression of EPO was correlated with Hb level in the control group in plateau (r =0. 83 , P < 0. 05). Conclusion Ershiwei Chenxiang Wan can decrease the levels of Hb and Hct in the rats exposed to hypobaric hypoxia, the mechanism may be reducing expression of EPO.