透明质酸联合羟乙基淀粉预防大鼠术后腹腔粘连的效果观察

摘要

目的 探讨透明质酸(HA)、羟乙基淀粉(HES)防粘连剂预防大鼠术后腹腔粘连的最佳剂量及其效果.方法 以HA、HES为原料制备不同浓度的防粘连剂；选择SD大鼠55只,随机分为阴性对照组(生理盐水)、阳性对照组(HA-羧甲基纤维素)、HA组(0.5％ HA和1.0％ HA)、HA+ HES组(1.0％ HA+ 0.03％ HES、1.0％ HA+0.06％ HES、1.0％ HA +0.12％ HES、1.0％ HA+0.24％ HES、1.0％ HA +0.50％ HES)；腹腔手术制备大鼠腹腔粘连模型后,分别注入不同浓度的防粘连剂,7d后观察各组的防粘连效果.结果 1.0％HA +0.50％ HES腹腔粘连面积为(0.06 ±0.10)cm2,粘连等级为Ⅰ级,达到理想防粘连效果.结论 1.0％ HA+ 0.50％ HES防粘连剂可明显预防腹腔粘连形成.%Objective To observe the effect of hyaluronic acid (HA) and hydroxyethyl starch (HES)on preventing SD rats postoperative adhesion and optimal dose. Methods HA and HES as raw materials were used to preparate the different concentrations of anti-adhesion agent; Selecting 55 SD rats were divided randomly into negative control group (saline) , positive control group ( HA-Carboxymethyl cellulose) , HA group (0.5% HA and 1.0% HA ) , HA + HES group (1.0% HA+0.03% HES, 1.0% HA+0.06% HES, 1.0% HA+0.12% HES, 1.0% HA+0.24% HES, 1.0% HA + 0.50% HES) ; After abdominal operation preparation of rat peritoneal adhesion model were injected into the different concentration of anti-adhesion agents and observed the effects of anti-adhesion agents at 7 d later. Results 1. 0% HA + 0.50% HES postoperative adhesion area was (0.06 ±0. 10)cm , adhesion grade I achieved the ideal effect of preventing adhesion. Conclusion 1.0% HA+0.50% HES anti-adhesion agent can obviously prevent the formation of postoperative adhesion.