扩张型心肌病患者外周血单个核细胞miR-155表达及意义

摘要

Objective To observe the expression changes of microRNA-155 ( miR-155) in the peripheral blood mono-nuclear cells ( PBMC) of patients with dilated cardiomyopathy ( DCM) and to explore the relationship between miR-155 level and heart function .Methods Thirty patients with DCM ( observation group ) and 30 healthy volunteers ( control group) were selected.In the observation group , 10 cases were grade Ⅱof NYHA functional classification , 11 cases were grade Ⅲand 9 cases were grade Ⅳ.PBMC in the two groups were separated by Ficoll-Hypaque density gradient centrifuga-tion, and the relative expression of miR-155 was detected by real-time fluorescence quantitative PCR .The miR-155 levels were compared between the observation group and the control group and among DCM patients with different NYHA function -al classification.In the observation group, the left ventricular ejection fraction (LVEF) and left ventricular end diastolic diameter ( LVEDd ) were recorded by color Doppler ultrasound .The relationships between miR-155 levels and LVEF , LVEDd were analyzed in DCM patients .Results The relative expression levels of miR-155 in the observation group and control group were (0.50 ±0.24) and (1.00 ±0.23), which showed a significant difference (P<0.01).The relative expression levels of miR-155 in NYHA functional classification Ⅱ,Ⅲ,ⅣDCM patients were (0.77 ±0.10), (0.47 ± 0.10) and (0.23 ±0.08), respectively.There were significant differences between each two groups (all P<0.01).In the observation group , the LVEF was 28%±10%, and the LVEDd was (70 ±7) mm.The miR-155 level was negatively correlated with NYHA functional classification , LVEF and LVEDd in DCM patients ( r=-0.94, -0.67 and -0.53, P<0.05 or P<0.01).Conclusions The expression level of miR-155 is down-regulated in PBMC of DCM patients and is correlated with the severity of impaired cardiac function .%目的：探讨微小RNA-155（miR-155）在扩张型心肌病（DCM）患者外周血单个核细胞（PBMC）中的表达变化及其与心功能的关系。方法选择DCM患者（观察组）及同期健康志愿者（对照组）各30例，观察组NYHA心功能Ⅱ级10例、Ⅲ级11例、Ⅳ级9例。采用Ficoll-Hypaque密度梯度离心法分离两组空腹静脉血中的PBMC，实时荧光定量PCR法检测PBMC中miR-155的相对表达量。比较观察组不同NYHA心功能分级者PBMC中miR-155的相对表达量，并分析其相关性。观察组同时行彩色多普勒检查，记录其左心室射血分数（ LVEF）、左心室舒张末期内径（LVEDd），分析两者与miR-155表达的相关性。结果观察组miR-155相对表达量为0．50±0．24，对照组为1．00±0．23；两组比较，P＜0．01。观察组NYHA心功能Ⅱ、Ⅲ、Ⅳ级者miR-155相对表达量分别为0．77±0．10、0．47±0．10、0．23±0．08，多组间及组内两两比较，P均＜0．01。观察组LVEF为28％±10％，LVEDd为（70±7） mm。观察组miR-155相对表达量与NYHA 心功能分级、LVEF、LVEDd 均呈负相关（ r 分别为－0．94、－0．67、－0．53，P＜0．05或＜0．01）。结论 DCM患者PBMC中miR-155表达降低，与患者心功能受损的严重程度有关。