Objective To investigate the effects of preoperative interstitial chemotherapy on the COX -2 expression and lymphatic vessel density ( LVD) in well-differentiated oral squamous cell carcinoma ( OSCC) .Methods Sixty specimens of OSCC were selected , in which 15 specimens were treated by preoperative interstitial chemotherapy with slow-released 5-FU ( interstitial chemotherapy group ) , 15 specimens underwent preoperative intravenous chemotherapy with pingyangmycin ( intravenous chemotherapy group ) and 30 specimens were not treated with chemotherapy ( non-chemotherapy group ) .The carcinomatous tissues and the tissues 3 cm from the carcinoma edge were embedded by paraffin .The expression of COX-2 and LVD marked with D2-40 in OSCC was detected by immunohistochemistry .Results The positive expression rates of COX-2 were respectively 40.0%, 66.6% and 83.3% in the interstitial chemotherapy group , intravenous chemotherapy group and non-chemotherapy group, and the difference among each group was significant (all P<0.05).The average LVD around the carcinoma were respectively (4.02 ±0.88), (8.12 ±0.79) and (12.22 ±2.25) vessels/mm2 in the intersti-tial chemotherapy group , intravenous chemotherapy group and non-chemotherapy group , and the difference among each group was significant (all P<0.05).In the non-chemotherapy group, 25 specimens with positive and 5 specimens with negative expression of COX-2, the average LVD around the carcinoma were respectively (12.91 ±1.66) and (8.75 ± 1.40) vessels/mm2, and significant difference was found between them (P<0.01).In the interstitial chemotherapy group and intravenous chemotherapy group , 16 specimens with positive and 14 specimens with negative expression of COX-2, the average LVD around the carcinoma were respectively (6.66 ±2.11) and (4.88 ±2.10) vessels/mm2, and significant difference was found between them (P<0.05).In the non-chemotherapy group, 14 specimens with lymph node metastasis and 16 specimens without lymph node metastasis , the average LVD around the carcinoma were respectively ( 13 .79 ± 1.76), (10.84 ±1.66) vessels/mm2, the positive expression rates of COX-2 in the carcinomatous tissues were respective-ly 100.0%and 68.8%, and significant difference was found in LVD around the carcinoma and the positive expression rate of COX-2 in the carcinomatous tissues between these two groups (all P<0.01).Conclusion Preoperative interstitial chemotherapy could reduce the expression of COX-2 and inhibit the lymphangiogenesis in well-differentiated oral squamous cell carcinoma .%目的 探讨术前间质化疗对高分化口腔鳞状细胞癌组织中环氧化酶2 ( COX-2 )表达及淋巴管密度(LVD)的影响. 方法 选择60例高分化口腔鳞状细胞癌患者,其中术前缓释氟尿嘧啶(5-FU)间质化疗者15例(间质化疗组)、术前平阳霉素静脉化疗者15例(静脉化疗组)、未化疗者30例(未化疗组) ,取肿瘤组织及距肿瘤边缘3 cm组织石蜡标本,免疫组化法检测3组癌组织中COX-2表达及D2-40标记的LVD. 结果 间质化疗组、静脉化疗组、未化疗组癌组织中COX-2阳性表达率分别为40.0%、66.7%、83.3%,三组间比较,P均<0.05. 间质化疗组、静脉化疗组、未化疗组癌周LVD分别为(4.02 ±0.88)、(8.12 ±0.79)、(12.22 ±2.25)支/mm2,三组间比较, P均<0.05. 未化疗组癌组织中COX-2表达阳性25例、阴性5例,癌周LVD分别为(12.91 ±1.66)、(8.75 ±1.40)支/mm2 ,二者比较,P<0.01;间质化疗组、静脉化疗组癌组织中COX-2表达阳性16例、阴性14例,癌周LVD分别为(6.66 ±2.11)、(4.88 ±2.10)支/mm2 ,二者比较,P<0.05. 未化疗组有淋巴结转移14例、无淋巴结转移16例,癌周LVD分别为(13.79 ±1.76)、(10.84 ±1.66)支/mm2 ,癌组织中COX-2阳性表达率分别为100.0%、68.8%,二者癌周LVD、癌组织中COX-2阳性表达率比较,P均<0.01. 结论 术前间质化疗能降低高分化口腔鳞状细胞癌组织中COX-2表达,抑制癌周淋巴管形成.
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