褪黑素对 PANC-1细胞增殖的影响及机制探讨

摘要

目的：观察褪黑素对人胰腺癌细胞（ PANC-1细胞）增殖的影响，并探讨其机制。方法取PANC-1细胞，以不同浓度褪黑素干预，采用MTT法检测PANC-1细胞增殖情况并计算增殖率，Western blotting法检测PANC-1细胞中Notch1的活性片段（NICD1）。结果对照1组细胞增殖率为0．38％±0．03％，褪黑素组加入1、1000、1000000 nmol／L的褪黑素后细胞增殖率分别为0．36％±0．02％、0．32％±0．02％、0．26％±0．01％，对照1组与褪黑素组加入1000、1000000 nmol／L的褪黑素后增殖率比较， P均＜0．05。对照2组细胞增殖率为0．43％±0．02％，DAPT组细胞增殖率为0．30％±0．02％，两组比较，P＜0．05。对照1组NICD1相对表达量为0．47±0．05，褪黑素组加入1、1000、1000000 nmol／L的褪黑素后NICD1相对表达量分别为0．32±0．04、0．33±0．05、0．21 ±0．03，对照1组与褪黑素组加入1000000 nmol／L的褪黑素后NICD1相对表达量比较，P均＜0．05。对照2组NICD1相对表达量为0．63±0．05，DAPT组NICD1相对表达量为0．38±0．03，两组比较，P＜0．05。结论褪黑素可以抑制PANC-1细胞的增殖，其机制可能是通过调节Notch信号通路来实现。%Objective To observe the influence of melatonin on the PANC-1 cell proliferation and to investigate its mechanism .Methods PANC-1 cells were treated with different concentrations of melatonin , and MTT method was used to detect the proliferation of PANC-1 cells and to calculate the proliferation rate .Western blotting was used to detect the ex-pression of active fragment of Notch 1 ( NICD1) in PANC-1 cells.Results The proliferation rate of the control group 1 was 0.38%±0.03%, after adding the concentration of 1, 1 000 and 1 000 000 nmol/L melatonin, the proliferation rates of PANC-1 cells were 0.36%±0.02%, 0.32%±0.02%and 0.26%±0.01%, respectively.Significant difference was found between the control group 1 and the melatonin groups with concentrations of 1 000 and 1 000 000 nmol/L melatonin (all P<0.05).The proliferation rate of the control group 2 was 0.43%±0.02%, the proliferation rate in the DAPT group was 0.30%±0.02%(P<0.05).The expression of NICD1 in the control group 1 was 0.47 ±0.05.After adding the concentrations of 1, 1 000 and 1 000 000nmol/L melatonin, the expression of NICD1 was 0.32 ±0.04, 0.33 ±0.05 and 0.21 ±0.03, respectively.Significant difference in the NICD1 expression was found between the control group 1 and the melatonin groups with concentrations of 1 000 and 1 000 000 nmol/L melatonin (all P<0.05).The expression of NICD1 in the control group 2 was 0.63 ±0.05.The expression of NICD1 in the DAPT group was 0.38 ±0.03 (P<0.05).Conclusion Melatonin can inhibit the proliferation of PANC-1 cells, whose mechanism may be involved in regula-ting the Notch signaling pathway .