肾癌组织 EphA2、E-cadherin 表达及其与肾癌临床病理特征的关系

摘要

Objective To investigate the expression of Eph receptor tyrosine kinase-A2 (EphA2) and E-cadherin in renal cell carcinoma ( RCC) and its relationship with clinical pathological features of patients .Methods The expression of EphA2 and E-cadherin was detected by immunohistochemical SP method in the tumor tissues from 52 RCC patients .The HSCORE score and percentage of positively stained cells were calculated .The relationships between the expression of E-phA2 and E-cadherin and clinical characteristics including age , gender , TNM classification , diameter of the tumor and pathologic types were analyzed .The three-year survival rate was calculated with the Kaplan-Meier method and compared by the Log-rank test.The main risk factors were screened by Cox hazard regression model .Results The percentage of pa-tients with high expression of EphA2 was 71.2%, and the percentage of patients with low expression of E-cadherin was 76.9%.The high expression rate of EphA 2 in patients with a tumor diameter ≥5 cm was higher than those with a tumor diameter <5 cm (P<0.05), and the low expression rate of E-cadherin in patients with a tumor diameter ≥5 cm was higher than those with a tumor diameter <5 cm (P<0.05).The high expression of EphA2 was a risk factor for the out-come of RCC patients (P<0.05).Conclusion The expression of EphA2 was increased, and the expression of E-cadher-in was decreased in the RCC tissue .The high expression of EphA 2 was the an independent risk factor for the prognosis of RCC patients .%目的：探讨Eph受体酪氨酸激酶A2（EphA2）及E-钙黏蛋白（E-cadherin）在肾癌组织中的表达及其与肾癌临床病理特征的关系。方法选择52例肾癌患者的手术标本，采用免疫组化SP法检测肾癌组织EphA2及E-cadherin蛋白的表达，计算每张切片的阳性细胞百分数及HSCORE分值。分析EphA2表达、E-cadherin表达与患者临床病理特征（包括年龄、性别、TNM分期、肿瘤直径、病理类型）的关系，计算3年生存率，应用COX比例风险模型分析肾癌患者预后影响因素。结果肾癌组织中EphA2蛋白高表达率为71．2％， E-cadherin 蛋白低表达率为76．9％。肿瘤直径≥5 cm者的EphA2蛋白高表达率高于直径＜5 cm者（P＜0．05），肿瘤直径≥5 cm者E-cadherin蛋白低表达率高于直径＜5 cm者（P＜0．05）。 EphA2蛋白高表达是肾癌患者预后的独立影响因素（P＜0．05）。结论肾癌组织中EphA2蛋白表达增加、E-cadherin蛋白表达降低，EphA2蛋白高表达是肾癌患者预后的独立影响因素。