95％二甲四氯原药短期重复染毒对大鼠NAG、BUN、Cr的影响

摘要

Objective:this experiment was established by intragastric administration of 95％2-methyl-4-chlorophenoxy acetic acid pesticides of short-term repeated exposed rats model, through the observation of each dose group rats organ coefficient, urinary creatinine (Cr), beta -N-Acetylglucosaminidase (NAG);Cr blood urea nitrogen (BUN);change renal NAG, to understand the effect of 95％ 2 -methyl -4 -chlorophenoxy acetic acid related indexes on rat kidney, 2 -methyl -4 -chlorophenoxy acetic acid is widely used in agricultural production to provide experimental basis.Methods:healthy adult SD 40 rats were randomly divided into,solvent control group,low dose group,middle dose group,high dose group 10 rats in each group, male and female.Half.With 10mL/kg.bw orally,for 28 days.We observed the meter kidney is the organ coefficient of rats;blood was detected by Cr,BUN by automatic biochemical analyzer;urine Cr test kit,NAG,renal NAG.results:The results showed that the renal index group comparison of female rat kidney coefficient of each dose group and the control,the difference was not statistically significant ( P>0.05);group kidney organ coefficient of male rats in each dose group with the control, the difference was not statistically significant (P>0.05).Urine NAG, Cr results showed that female rats of urinary NAG in each dose group compared with the control group,the difference was statistically significant (F=6.774, P<0.05);group of male rat urine NAG high dose group and the control,the difference was statistically significant (P<0.05);group of female rat urine Cr in each dose group and the control,the difference was not statistically significant(P>0.05);group of male rat urine Cr in each dose group and the control,the difference no statistical significance (P>0.05).The levels of BUN, Cr showed that female rats blood in Cr group compared with the control group,the difference was statistically significant (P<0.05);group of female rats with different doses of BUN blood group and the control,the difference was not statistically significant ( P>0.05);group of male rats with different doses of BUN blood group and the control,the difference was not statistically significant (P>0.05);group of male rats with different doses of Cr blood group and the control, the difference was not statistically significant ( P>0.05) .Renal NAG showed that renal NAG male rats in dose group, high dose and control group.The difference was statistically significant ( P<0.05 );group of female rats in each dose group of renal NAG and the control,the difference was not statistically significant (P>0.05).The results showed that the correlation relationship between urinary NAG and renal NAG (r=0.341,P<0.05);urinary Cr and NA The relationship between G ( r =0.374, P<0.05 );there is correlation between urine Cr and blood Cr ( r =0.379, P<0.05 ) .Conclusion:95％2-methyl-4-chlorophenoxy acetic acid a drug can lead to Cr,BUN,NAG changes,causing damage to the kidney function of rats.The changes of the urinary Cr value,can understand the short-term repeated contact 95％2-methyl-4 -chlorophenoxy acetic acid pesticide in kidney damage.Therefore in agricultural production, should avoid long -term exposure to 95％2-methyl-4-chlorophenoxy acetic acid a TC.%目的:采用灌胃法建立95％二甲四氯原药短期重复染毒大鼠模型,通过观察各剂量组大鼠肾脏脏器系数、尿肌酐(Cr)、β-N-乙酰氨基葡萄糖苷酶(NAG);血Cr、尿素氮(BUN);肾NAG的变化,了解95％二甲四氯原药对大鼠肾脏相关指标的影响,为二甲四氯广泛应用于农业生产提供实验依据.方法:SD成年健康大鼠40只,按体重随机分为溶剂对照组、低剂量组、中剂量组、高剂量组,每组10只,雌雄各半.以10mL/kg.bw灌胃,连续28天.染毒结束后,计算大鼠肾脏脏器系数;采用全自动生化分析仪检测血Cr、BUN;采用试剂盒检测尿Cr、NAG、肾NAG.结果:肾脏脏器系数结果显示:雌鼠肾脏脏器系数各剂量组与对照组比较,差异无统计学意义(P>0.05);雄鼠肾脏脏器系数各剂量组与对照组比较,差异无统计学意义(P>0.05).尿NAG、Cr结果显示:雌鼠尿NAG各剂量组与对照组比较,差异有统计学意义(F=6.774,P<0.05);雄鼠尿NAG高剂量组与对照组比较,差异有统计学意义(P<0.05);雌鼠尿Cr各剂量组与对照组比较,差异无统计学意义(P>0.05);雄鼠尿Cr各剂量组与对照组比较,差异无统计学意义(P>0.05).血BUN、Cr结果显示:雌鼠血Cr中剂量组与对照组比较,差异有统计学意义(P<0.05);雌鼠血BUN各剂量组与对照组比较,差异无统计学意义(P>0.05);雄鼠血BUN各剂量组与对照组比较,差异无统计学意义(P>0.05);雄鼠血Cr各剂量组与对照组比较,差异无统计学意义(P>0.05).肾NAG结果显示:雄鼠肾NAG中剂量组、高剂量组与对照组比较,差异有统计学意义(P<0.05);雌鼠各剂量组肾NAG与对照组比较,差异无统计学意义(P>0.05).相关性结果显示:尿NAG和肾NAG有相关性(r=0.341,P<0.05);尿Cr和尿NAG有相关性(r=0.374,P<0.05);尿Cr和血Cr有相关性(r=0.379,P<0.05).结论:95％二甲四氯原药可导致Cr、BUN、NAG的改变,对大鼠肾脏功能造成损伤.通过检测尿Cr值的变化情况,可了解短期重复接触95％二甲四氯原药对肾脏的损伤情况.在农业生产中,应避免长期接触95％二甲四氯原药.