一种适合杨树树皮的蛋白质提取方法

摘要

双向电泳技术是进行蛋白质组分分离和功能鉴定的重要步骤.提取和制备高质量的蛋白质是进行双向电泳的首要前提.杨树树皮组织中含有大量的干扰物质,影响了蛋白质的提取和双向电泳的分离效果.为建立一种适合杨树树皮蛋白质的双向电泳程序,本实验以107杨为材料,采用改良的三氯乙酸-丙酮沉淀法提取杨树树皮蛋白质,经双向电泳和考马斯亮蓝染色检测,得到了理想的双向电泳图谱,为杨树与病原互作的蛋白质组学研究奠定了基础.%Two - dimensional electrophoresis ( 2 - DE) is an important step for protein separation and function identification. Extracting and preparing high - quality protein is the first premise of 2 - DE. There is a large amount of interfering substances in poplar bark tissues, which influence the extraction of protein and separation effect of 2 - DE. In order to develop a 2 - DE procedure suitable for poplar bark protein, the poplar 107 was used as material. The improved method of trichloroacetic acid (TCA) - acetone was adopted to extract the proteins of poplar bark. After the two - dimensional electrophoresis and detection with Coomassie brilliant blue, the desirable gel maps were obtained. It layed the foundation for proteomics research of poplar and pathogen interaction.