花生赤霉素2-氧化酶基因的克隆和表达研究

摘要

赤霉素2-氧化酶(GA2ox)是赤霉素合成过程中起负调控作用的一种关键酶,催化有活性的赤霉素生成无活性的赤霉素.从花生荚果发育不同时期的转录组序列中筛选到GA2ox的基因片段,采用5’-RACE方法克隆了花生GA2ox基因的全长cDNA序列.序列分析表明,花生GA2ox蛋白氨基酸序列含有和其他植物同样保守的结构域.通过qRT-PCR技术对GA2ox基因在花生不同组织器官中的表达分析,发现所检测的10种组织中均有GA2ox的表达,其中成年叶片中表达最丰富,根、刚入土的果针次之.本研究结果为进一步分析该基因在花生生长发育过程中的作用提供了有用信息.%Gibberellin 2 -oxidase (GA2ox) is a key enzyme converting bioactive gibberellins to nonac-tive gibberellins during gibberellin biosynthesis. The GA2ox fragment was identified from transcriptome sequences , and its full length cDNA was acquired by RACE amplification. The sequence alignment showed that the amino acid sequence of peanut GA2ox contained the same conserved domains as that from other plant species. The quantitative RT - PCR results showed that AhGA2ox was expressed in all the 10 examined tissues; the highest expression level was detected in leaves, and that in root and pegs was second. This study provided useful information for further research of the functions of this gene during peanut growth and development.