微生态制剂联合肠内营养对重症急性胰腺炎大鼠肠运转功能的影响

摘要

Objective: To investigate the influences of probiotics、 prebiotics and synbiotics supplement with enteral nutrition on the intestinal transfer of rats with acute severe pancretitis. Methods:Thirty Sprague-Dawley (SD) model rats of acute severe pancretitis , which had be administerated with enteral nutrition plus with prebiotics or probiotics 7 days were divided into five groups : EN group ( A), prebiotics+EN group ( B) ,probiotics+EN group ( C) ,synbiotics + EN group ( D) and control group (E)via jejunostomy . The nutrition support in the five groups was isonitrogen and isocaloric. Before sacrificed on the seventh day ,gastrointestinal transfer test was performed and vein blood was tested for MTL; Specimens of jejunum were obtained to measure the ATPase activity. Results: gat transfer index、the level of serum MTL decreased significantly in E group,the quantity of ATPase in gastrointestinal tissue decreased mostly in E group than other group. Compared with B group ,the levels of gat transfer index、Mg2+-ATPase、Ca2+-Mg2+-ATPase in C group were higher. The level of serum MTL、Na+-K+-ATPase 、Ca2+ -ATPase was significantly higher in C groups than those in A and B group. although gat transfer index 、the level of serum MTL and gastrointestinal tissue ATPase activity increased in B and D group , there are no differences between synbiotics +EN group,prebiotics+EN group and E groups. Conclusion : Our results suggested that probiotics and EN can enhance the function of small intestinal peristalsis of SAP rat by promoting secretion of motilin and activity of ATPase while β-glucans can not.%目的:探讨微生态制剂培菲康、燕麦β-葡聚糖联合肠内营养对重症急性胰腺炎大鼠胃肠的运动及吸收功能的影响.方法:将30只大鼠诱导重症胰腺炎模型后,随机分为5组,分别给予肠内要素营养(A组)、β-葡聚糖+肠内要素营养(B组),培菲康+肠内要素营养(C组),培菲康+β-葡聚糖+肠内要素营养,即合生元+肠内要素营养(D组)以及模型对照组(E组),共持续7d.5组营养供给为等热、等氮量.第7d处死大鼠,测肠运转系数,取血检测血浆MTL的变化,取空肠观察肠组织ATP酶的含量.结果:E组大鼠的肠运转系数、血浆MTL水平显著下降,而肠酶活性大部分低于各试验组,差异有统计学意义;C组肠运转系数、Mg2+-ATPase、Ca2+-Mg2+-ATPase活性水平显著高于其他各组,与B组差别显著;C组的血浆MTL水平、肠Na+-K+-ATPase、Ca2+-ATPase显著高于A组及B组,差别有显著性;B组、D组肠运转系数、血浆MTL水平及肠酶活性虽然高于E组,但无统计学意义.结论:益生菌联合肠内营养可促进重症急性胰腺炎大鼠胃肠运转功能;益生元及合生元联合肠内营养对重症急性胰腺炎大鼠胃肠运转功能改善不明显.