白癜风皮肤组织角质形成细胞释放警报素分子的研究

摘要

Objective :To investigate the release of HMGB1 by HaCaT cell and keratinocytes of tissue from vitiligo patients .Methods :HaCaT cells were treated with UV light or with apoptosis drugs or with cytokines and freeze-thaw process . The supernatant was condensed and subjected to SDS-PAGE to detect the release of HMGB1 .Measure the HMGB1 and cleaved caspase-3 expression in skin biopsies of normal control subjects and viti-ligo patients by immunofluorescence .Results :HMGB1 was detected in the supernatant of HaCaT after treated and control supernatant was not detected .Cleaved caspase-3 was found in the stratum corneum of vitiligo patients and control patient was not found .HMGB1 was expressed in the nucleus of keratinocytes of control patients and trans-ferred from nucleus to cytoplasm in vitiligo patients .Conclusion:HMGB1 can be passively released by apoptosis and necrosis keratinocytes or actively released in vitiligo .%目的：探讨人永生化角质形成细胞（HaCaT ）和人白癜风组织表达与释放高迁移率蛋白B1（HMGB1）。方法：HaCaT分别经紫外线照射、加凋亡诱导剂、细胞因子刺激、反复冻融处理，细胞培养上清经浓缩后，Western-blot观察24h后 HMGB1的释放。免疫荧光染色检测白癜风患者和对照组的活检皮肤组织 HMGB1和激活的半胱氨酸蛋白酶3（cleaved caspase-3）的表达。结果：HaCaT经各种处理后，培养上清中均能检测出 HMGB1，对照组则不能。白癜风病人角质层检测到cleaved caspase-3，对照组病人未检测到；对照组HMGB1表达在角质形成细胞核内，白癜风组织H M GB1从细胞核转移至细胞浆。结论：H M GB1能够由角质形成细胞在凋亡和坏死时被动释放或者在病理状态下（白癜风）主动分泌。