α-硫辛酸对溃疡性结肠炎小鼠Th17相关炎症因子表达影响研究

摘要

目的：探讨α-硫辛酸（α-lipoic acid ，ALA）对溃疡性结肠炎小鼠Th17相关炎症因子表达的影响。方法：将雄性Balb／c小鼠（22～25 g）随机分为对照组（Control）、ALA对照组（ALA）、溃疡性结肠炎组（UC）、ALA 治疗组（UC＋ ALA）四组，每组10只，观察各组小鼠疾病活动指数（DAI）评分，比较结肠质量指数及结直肠组织病理变化；酶联免疫吸附（ELISA ）检测小鼠血清中IL-17、IL-21分泌情况，RT-PCR检测结肠组织 TNF-α、TGF-β mRNA 表达情况。结果：结肠质量指数、DAI及组织病理学评分在对照组及ALA组中无明显差异，但在UC组中显著升高，在UC＋ALA组中明显降低（P＜0．05）；IL-17、IL-21在对照组及ALA 组小鼠血清中的水平无明显差异，但在UC组中显著升高，在UC＋ALA组中明显降低（P＜0．05）；TNF-α、TGF-β mRNA 在对照组及ALA组小鼠结肠组织中的表达水平无明显差异，但在UC组中显著升高，在UC＋ ALA组中明显降低（P＜0．05）。结论：α-硫辛酸能够降低 TNBS引起的溃疡性结肠炎小鼠IL-17、IL-21、TNF-α、T G F-β等T h17相关炎症因子的表达水平，改善炎症反应及病理损伤。%Objective:To investigate the effect of α-lipoic acid (ALA) on the expression of Th17 related inflammatory factors in mice with ulcerative colitis .Methods:The Balb/c mouse model with ulcerative colitis was induced by three sulfonic acid trinitrobenzene (TNBS) .Male Balb/c mice (22~25 g) were divided into four groups , with control group (Control) ,ALA control group (ALA) ,ulcerative colitis group (UC) ,and ALA in the treatment of ulcerative colitis group (UC+ ALA) ,10 mice in each group .The colonic mass index ,the disease activity index (DAI) and the pathological score of HE (HDS) in each group were calculated .The secretion of IL-17 and IL-21 in mice serum was detected by enzyme linked immunosorbent assay (ELISA) .The autophagy gene TNF-α,TGF-βmRNA level was detected by RT-PCR .Results :There was no significant difference between Control group and ALA group in the quality index ,DAI and histopathology score ,but it was significantly increased in the UC group , and significantly lower in the UC+ALA group (P<0 .05) .There was no significant difference between IL-17 and IL-21 in Control group and ALA group of mice serum levels ,but increased significantly in the UC group ,and signif-icantly lower in the UC+ ALA group (P< 0 .05) .There was no significant difference in the expression levels of TNF-α,TGF-βmRNA and in Control group and ALA group ,but significantly increased in the UC group ,and sig-nificantly lower in the UC+ ALA group (P<0 .05) .Conclusion:Theα-lipoic acid can reduce the expression levels of IL-17 ,IL-21 ,TNF-α,TGF-βand other Th17 related inflammatory factors in ulcerative colitis mice induced by TNBS ,and improve the inflammation ,as well as promote pathological damage repair .