护胃散对急进高原大鼠胃黏膜血管内皮生长因子、碱性成纤维生长因子水平的影响及其机制探讨

摘要

Objective:To study the effect of Huwei powder on the levels of VEGF and bFGF ingastric mucosa of radical plateau rats and its mechanism .Methods :Choose Wistar rats ,randomly divided intoBeijing group ,radical plateau groups(Xining group ,Maduo group) ,of which ,Maduo group sets up blank control group ,positive control group ,Huwei powdergroups .Beijing group ,Xining group ,blank control group of Maduo give the physiological saline , positive control group gives Jinghua Weikang capsules and Huwei powder groups (high ,medium and lowdoses) ,and each group respectively obtains rats' gastric mucosain1 d ,2 d ,3 d ,5 d ,7 d ,and the levels of VEGF and bFGF were detected by ELISA method .Results :The study shows that with the increase of altitude and the extension of time ,the rats' gastric mucosa levels of VEGF and bFGF increased .Compared with Beijing group ,Huwei powder could effec-tively increase the levels of VEGF and bFGF ingastric mucosa of acute rats (P<0 .05 or P<0 .01) .Conclusion :Hu-wei powder can effectively improve the levels of VEGF and bFGF ingastric mucosa of radical plateaurats ,and plays an important role in repair of acute gastric mucosal injury .%目的:探讨护胃散对急进高原大鼠胃黏膜血管内皮生长因子(VEGF)、碱性成纤维生长因子(bFGF)的水平影响及作用机制.方法:选用Wistar大鼠,随机分为北京组、急进高原组(西宁组、玛多组),其中,玛多组设空白对照组、阳性药组、护胃散组.北京组、西宁组、玛多空白对照组给予生理盐水,阳性药组给予荆花胃康胶丸及护胃散组(高、中、低剂量),各组分别于第1天、2天、3天、5天、7天取大鼠胃黏膜,并用ELISA法测定VEGF、bFGF水平.结果:研究表明,随着海拔的升高、时间的延长,大鼠胃黏膜中VEGF、bFGF的水平不断增加;与北京组比较,护胃散能有效提高急进高原大鼠胃黏膜VEGF、bFGF的水平(P<0.05或P<0.01).结论:护胃散能有效提高急进高原大鼠胃黏膜VEGF、bFGF的含量,在急性胃黏膜损伤修复方面发挥着重要作用.