介绍了微束装置的主要工作原理,并用2.0 MeV、25μm左右束斑的离子微束,以6×106ions的剂量对无芒雀麦种胚的茎尖部位和根尖部位进行了定点定量注入,详细研究了处理后胚根的生长发育,如主根的伸长、侧根的形成等.结果表明,根的生长发育过程受到了离子注入的显著影响,由实验结果推测,根尖和茎尖分生组织与主侧根的发育均有关系,但所起的作用各不相同,此结果为进一步研究相关的发育调控机理和信号转导方式提供了基础.%The article describe operating principle of the single-particle microbeam device, we implanted the SAM and RAM of embryos with a 2. 0 MeV of energy and 25 μm of diameter proton microbeam at the fluence of 6 × 106 ions. The embryos' root patterning parameters was observed in detail, such as the development of primary root and the formation of lateral root. The results indicated that some contributions of vacuum treatment might be involved in the biological effects, ion-beam bombardment and the interactions might be existed between each other. The results showed that auxin signal transduction might be relevant.
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