青钱柳茎段腋芽萌发和丛生芽增殖

摘要

To establish an efficient tissue culture system for rapidly propagating Cyclocarya paliurus, axillary buds from the stem segment were used as explants and cultured on WPM medium.Effects of the different types and concentration of plant growth regulator on the germination of axillary buds and proliferation of multiple shoots were studied.The results showed that all nine hormone combinations promoted germination of axillary buds to some degree, among which the most suitable hormone combination was 6-BA 3 mg·L-1 +2ip 1 mg· L-1 + NAA 0.1 mg·L-1 , with 83.33％ germination rate,the best growth state, but inducing only single bud in every treatment.Multiple shoots could be induced when the single bud was cultured in medium with TDZ, the optimal hormone combination was TDZ 0.1 mg· L-1 + NAA 0.1 mg·L-1,and the germination rate and multiplication coefficient reached up to 100％ and 7.33 respectively.With addition of 2.0 mg· L-1 GA3 , the elongation of multiple shoots was efficiently improved, and the shoots were healthy and strong, which provided a good foundation for rooting later.The study provided a basis for establishing rapid propagation system for Cyclocarya paliurus with tissue culture, and provided technical reference for developing rapid propagation of other forest species.%为建立高效的青钱柳组培快繁体系,以青钱柳茎段腋芽作为外植体,WPM为基本培养基,研究不同植物生长调节剂种类及其浓度组合对腋芽萌发和丛生芽增殖的影响.结果表明:9个处理的激素组合对青钱柳腋芽萌发均有不同程度的促进作用.其中,6-BA 3 mg·L-1+2ip 1 mg·L-1 +NAA 0.1 mg·L-1处理效果最佳,萌芽率最高(83.33%),芽生长状态最好,但各处理诱导的均以单芽为主.将单芽置于添加TDZ的培养基中则诱导的均为丛芽,最佳组合为TDZ 0.1 mg·L-1+NAA 0.1 mg·L-1,萌芽率、增殖系数均达最高,分别为100%和7.33.培养基中添加2.0 mg·L-1 GA,能有效地促进丛生芽的伸长生长,芽苗生长健壮,为后续生根奠定良好基础.