基于28S rDNA基因的天牛科部分种类的分子系统发育

摘要

对天牛科4亚科32种天牛的28S rDNA基因部分序列进行测定和分析,并结合GenBank数据库中6种天牛的28S rDNA基因部分序列,采用最大简约法和贝叶斯法重建天牛科7亚科38种天牛的系统发育树,探讨它们之间的系统发育关系.在获得的772 bp的序列中,保守位点521个,占全部位点的67.5％;简约信息位点136个,占全部位点的17.6％.G+C的平均含量为60.8％,明显高于A+T的平均含量,碱基组成偏向G和C,转换稍高于颠换.系统发育树表明:天牛亚科、锯天牛亚科、花天牛亚科及沟胫天牛亚科均为单系群,与传统形态分类结果一致.28S rDNA序列是一种有效的解析天牛科高级分类阶元系统发育关系的分子标记.%Phylogenetic relationships among 38 species in 7 subfamilies of Cerambycidae were investigated using partial ribosomal 28 S rDNA sequence data. Phylogenetic trees were reconstructed using Maximum Parsimony methods and Bayesian Inference method. Sequence analysis indicated that there were 521 conserved sites, accounting for 67. 5% of all sites, and 136 parsimony informative sites, accounting for 17. 6% of all sites. The average content of G + C was 60. 8% , significantly higher than that of A + T, showing that the base compositions were biased in G + C. Transition was slightly higher than transversion. Phylogenetic tree results indicated that Cerambycinae, Prioninae, Lepturinae and Lamiinae were monophyletic group. This result was consistent with the traditional typoiogical classification. These results suggest that nuclear 28S ribosomal DNA would be a viable genetic marker to resolve divergences among subfamilies of Cerambycidae.