为获取栗疫病生物防治的基础信息,本研究根据菌株在PDA培养基上培养7天后的菌丝生长速度、分生孢子形成能力等培养特性,从韩国国立山林科学院树木病理研究室保藏的60个栗疫病菌菌株中筛选了2个弱致病力菌株,进行dsRNA检测、弱致病力菌株和强致病力菌株间的细胞融合试验.结果表明:2个弱致病力菌株(KCP-135和KCP-136)中均检测到了dsRNA,弱毒性菌株KCP-22和其他19个强毒性菌株之间的菌落形成明显的隔离带并沿着隔离带产生分生孢子,没有明显的细胞融合现象,而弱毒性菌株KCP-22和强毒性菌株KCP-9之间的菌落则呈现了显著的细胞融合现象,而且其细胞融合菌株的培养特性和转化dsRNA数量均发生了变异.%Chestnut blight disease caused by Cryphonectria parasitica is widely distributed throughout chestnut tree plantations in the world. Two hypovirulent strains were selected from 60 strains of C. parasitica by abnormal morphology detection such as reduced conidiation, reduced mycelial growth on PDA medium, and colony color on Bavendamm's medium. Double stranded RNA( dsRNA) was detected from the two hypovirulent strains. The vegetable compatibility group test of twenty virulent strains and one hypovirulent strain ( KCP-22 ) was conducted, and the results showed that only hypovirulent strain KCP-22 and virulent strain KCP-9 could be vegetable compatibility, and the amount of transmitted dsRNA and culture characteristics of the vegetable compatibility strains were changed.
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