培养基成分对雷公藤不定根生长和次生代谢产物含量的影响

摘要

以NT为基本培养基,以雷公藤不定根为材料,研究NT培养基中氮总量、硝态氮和铵态氮之比、磷酸盐、钾盐、钙盐、镁盐、铁盐浓度对雷公藤不定根生长及雷公藤甲素和总生物碱含量的影响.结果表明:适合雷公藤不定根生长的培养基中氮含量为NT培养基的1.7倍,N03-/NH4+为9:1,PO43-浓度、K+浓度、Ca2浓度、Mg2+浓度为NT培养基的2倍,Fe2浓度不变.NT培养基中原有的Mg2浓度、总氮浓度及其比例,1.7倍的PO43-浓度,1.3倍的K+浓度,2/3的Ca2+浓度,1/3的Fe2+浓度比较适合雷公藤甲素的积累.而对雷公藤总生物碱的积累各成分的最佳浓度为:NT培养基中原有的P034-,K+,Ca2+,Mg2+浓度,将硝态氮全部换成铵态氮以及1/3的Fe2+浓度.%In this paper the adventitious root of Tripterygium wilfordii was used as experiment material to study effects of different components in NT medium on the growth,triptolide and total alkaloid contents of the adventitious roots.The components include total nitrogen,the ratio of nitrate nitrogen and ammonium nitrogen,and different concentrations of PO43-,K+,Ca2+,Mg2+,Fe2+ etc.Additionally,this study was also to explore the change patterns of secondary metabolites and total alkaloid of the adventitious roots.The results showed that the optimal NT medium composition for the root growth was 1.7 strength of nitrogen,9:1 of NO3-/NH4+,and 2 strengths of PO~-,K +,Ca2+ and Mg2+,without change in Fe2+ conentration.The basic NT Mg2+ concentration,total nitrogen concentration and the NO3-/NH4+ ratio,1.7 strength of PO43-,1.3 strength of K +,2/3 strength of Ca2+,and 1/3 strength of Fe2+ were relatively favorable for the accumulation of triptolide.As for the accumulation of total alkaloids,the optimal component composition was the basic NT PO43-,K +,Ca2+ and Mg2+ concentration,but completely replacing the nitrate nitrogen with ammonium nitrogen,and 1/3 strength of Fe2 +.