应用nanoLC-MS/MS分析毛白杨次生维管系统的蛋白质表达谱

摘要

Abstract:As the uppermost carbon pool and important renewable source,wood has dual functions in both responding to the global climate change and meeting the resource demands of human society,thereby it is essential to understand the molecular mechanism of wood formation.In this study,a 1DE-based nanoLC-MS/MS method was introduced to analyze the proteome in the secondary vascular system of Populus tomentosa,and the identified proteins included:14 proteins associated cell division; 7 proteins related to transcription factors; 22 proteins associated with cytoskeleton; 31 proteins associated with signal transduction;and 14 proteins associated with cell wall formation.The corresponding genes of these proteins participate in regulating cambial activity.The accomplishment of this proteome analysis laid a foundation for further understanding the molecular mechanism of wood formation.%基于1DE的nanoLC-MS/MS方法分析毛白杨次生维管系统的蛋白质组,成功鉴定:14个蛋白质参与细胞分裂,7个转录因子,22个与细胞骨架相关蛋白质,31个与细胞信号转导相关蛋白质,14个与细胞壁相关蛋白质.显示这些蛋白质相应基因参与形成层活动的调控,为进一步了解木材形成的分子机制奠定基础.