超数排卵效果不同绵羊卵巢卵泡促卵泡素受体的分布

摘要

[目的]探讨在具有不同超数排卵效果的绵羊品种中,进行超排处理后其卵巢上促卵泡素受体(FSH receptor,FSHR)的分布情况,为进一步研究二者的相关性及据此改进超排效果奠定基础.[方法]根据超数排卵效果优劣依次选择3个中国地方品种的绵羊:小尾寒羊、白滩羊和乌珠穆沁羊.在常规超排最后一次注射FSH后的8、16和24 h分别各取3只绵羊的卵巢,并采用免疫组织化学法观察3种绵羊卵巢上FSHR的分布情况.[结果]FSHR主要分布于3个品种绵羊卵巢的颗粒细胞上;小尾寒羊最后一次注射FSH后8、16和24 h的各时期卵泡中FSHR的表达都显著高于另外两个品种绵羊(P白滩羊>乌珠穆沁羊,表明绵羊超数排卵效果与其卵巢上FSH受体分布存在一定联系.%[Objective]This study was aimed to investigate the distribution of FSHR (FSH receptor) on the ovaries among different local breeds of sheep by different efficiency of superovulation, and to do some preparations for further improving this ovine propagation technique.[Method]Three local ovine breeds were selected depending on their different own sensitivity range to superovulation by FSH as following: Small Tailed Han sheep, White Tan sheep, and Ujumqin sheep.At 8, 16, and 24 hour after the last injection with FSH, the ovine ovaries were collected and treated by immunohistochemical staining to observe the distribution of FSHR on their different ovarian follicles.[Result]The results showed that FSHR was expressed mainly on granulosa cells.At 8, 16,and 24 hour after the last injection with FSH, the FSHR levels on the ovarian follicles of Small Tailed Han sheep, were significantly higher than those of the other two breeds, while the FSHR levels in early and middle ovarian follicle of White Tan sheep, were also higher than Ujumqin sheep (P＜0.05).At 8, 16, and 24 hour after the last injection of FSH, the FSHR levels in the late ovarian follicle of those three breeds were significantly higher than those in the early and middle ovarian follicle (P＜ 0.05 ).The results showed that the responsive orders to FSH treatment of these three different local ovine breeds be one by one as following: Small Tailed Hah sheep＞White Tan sheep＞Ujumqin sheep.[Conclusion]It can be concluded that there is a relation between the effect of superovulation among different local sheep breeds and the distribution of FSH receptor on their ovaries.