可感染茶尺蠖的重组家蚕核型多角体病毒的构建

摘要

[Objective] The objective of this study is to construct recombinant BmNPV infecting both silkworm and Ecotropis oblique, and to solve the reproduction difficulty of EoNPV because of the breeding complexity. [Method] Recombinant BmNPV containing the polh expression box of BmNPV and the hel expression box of EoNPV were constructed by Bac-to-Bac system. [Result] rBmNPV amplified from silkworm or BmN cell could infect E. oblique, which indicated the host domain of rBmNPV had been broaden to E. oblique. [Conclusion] rBmNPV which could infect E. oblique was successfully constructed, thus laied a foundation of producing recombinant virus pesticide using silkworm as host.%[目的]构建能够感染家蚕和茶尺蠖的重组家蚕核型多角体病毒(rBmNPV),解决EoNPV扩增困难而不易获得的难题.[方法]通过Bac-to-Bac系统构建具备BmNPV的多角体蛋白基因(po1h)表达盒和EoNPV的解旋酶基因(he1)表达盒的重组家蚕核型多角体病毒.[结果]家蚕或家蚕细胞扩增的rBmNPV可以感染茶尺蠖并致病,表明rBmNPV的宿主域拓展至茶尺蠖.[结论]成功构建了能够感染茶尺蠖的rBmNPV,为利用家蚕作为宿主来生产重组病毒杀虫剂打下基础.