鸡胸腺重和脾脏重性状的全基因组关联

摘要

[目的]全基因组关联分析(genome-wide association study,GWAS)是复杂性状和疾病相关基因定位的新策略.[方法]试验利用鸡60K SNP芯片对来自50个公鸡家系的728只北京油鸡进行SNP分型检测,采用全基因组关联分析方法对影响100日龄胸腺重和脾脏重的基因进行定位研究.[结果]结果发现24个达5％全基因组水平显著的位点,与100日龄胸腺重和脾脏重显著相关,并在这些位点附近发现Janus kinase 1(JAK1)、zinc finger DHHC-type containing 8(ZDHHC8)、vav 3 guanine nucleotide exchange factor(VAV3),SATB homeobox 1(SATB1)等候选基因；84个与这两个性状潜在关联同时达到5％染色体水平显著的位点.[结论]利用GWAS分析策略筛选和鉴定的重要突变位点及候选基因,将为揭示鸡免疫器官发育的分子调控机制和抗病育种分子标记辅助选择提供必要的分子基础.%[Objective] Genome-wide association study (GWAS) is a new strategy to identify genes and chromosome regions associated with complex traits. [Method] A total of 728 Beijing-You chickens from 50 families were genotyped by 60K SNP Illumina iSelect chicken array. GWAS was performed to study the candidate loci affecting thymus weight and spleen weight at 100 days of age. [Result] It was found that 24 SNPs were significantly associated with target traits at Bonferroni corrected 5% genome-wise threshold, including Janus kinase 1 (JAK1), zinc finger DHHC-type containing 8 (ZDHHC8)、 vav 3 guanine nucleotide exchange factor (VAV3), SATB homeobox 1 (SATB1) and other genes near these SNPs, and 84 SNPs reached both the significance of 'suggestive linkage' and 5% chromosome-wise threshold. [Conclusion] These indentified genes and SNPs offered essential information for understanding of the molecular mechanisms of immune organs development and facilitated the application of marker-assisted selection in diseases-resistance breeding program in chicken.