不同氨基酸模式对奶牛乳腺上皮细胞酪蛋白合成影响的研究

摘要

该研究旨在验证氨基酸模式的不同是否会影响奶牛乳腺上皮细胞酪蛋白的合成，并对其机理进行初步探讨。试验采用完全随机试验设计，氨基酸模式分别为：低蛋白日粮条件下血液氨基酸组成模式组(LPBP)、全乳蛋白氨基酸组成模式组(MP)、酪蛋白氨基酸组成模式组(CP)、80%酪蛋白+20%乳清蛋白氨基酸组成模式组(CLP)，每个处理3个重复，试验重复3遍（n=12）。用RT-qPCR检测CSN1S1、CSN3、GLUT1、PRLR、STAT5和mTOR基因表达量，用Elisa法检测αS-酪蛋白合成量，用CCK-8检测细胞增殖活性。试验结果显示：氨基酸模式的不同影响奶牛乳腺上皮细胞酪蛋白的合成，全乳蛋白模式可能是一种较为理想的氨基酸模式。氨基酸模式通过调节mTOR、STAT5、PRLR和GLUT1基因的表达影响酪蛋白的合成。此外，研究发现在体外培养条件下，乳腺上皮细胞增殖活性与乳蛋白基因表达的趋势并不一致。%The objective of this study was to determine whether different amino acid (AA) patterns could affect casein synthesis in bovine mammary epithelial cells. A completely random design was used. Four AA patterns reatments are as follows: blood AA pattern in low protein diet (LPBP); milk protein AA pattern (MP); 80% casein plus 20% lactoalbumin AA pattern (CLP); casein AA pattern (CP). Each treatment has three replicates, and all experiments were repeated three times. The expression of CSN1S1, CSN3, GLUT1, PRLR, STAT5, mTOR genes and the content of αS-casein were determined by RT-QPCR method and αS -casein ELISA Kit, respectively.Cell proliferation was determined by CCK-8 method.The results showed that: The milk protein AA pattern might increase major milk protein genes expression. Perhaps, an appropriate AA pattern can promote mTOR STAT5 PRLR GLUT1 genes expression, and result in increased milk protein synthesis. In addition, the level of geng expression trend was different from the proliferation in mammary gland epithelial cell.