油杆菌W3分离自江苏油田韦5井,能够以稠油为唯一碳源生长,并降解稠油.54 ℃好氧振荡培养7 d之后,稠油能够完全乳化分散到培养基中,降黏率达到了76.88％,降解速率达到了99.7 mg/d,原油族组分分析发现,稠油中饱和烃,芳香烃和胶质的含量分别降低了4.14％,6.77％和4.33％.该菌与另一株筛选得到的、能够合成乳化剂的地芽孢杆菌W12复配(VW3:VW12=1:1)后作用稠油,既能够增强稠油降解效果(降解速率加快,原油族组分含量进一步降低),又能够改善稠油的乳化分散状态(乳化稳定性增强,平均乳化粒径减小56.7％),此时的稠油的乳化黏度为20.59 MPa·s,仅为初始黏度的3.9％.这两株菌的复配增强了稠油降解和乳化的双重降黏效果,大幅提高了稠油的流动性.%Petrobacter sp.W3 which could utilize heavy oil as the sole carbon source was isolated from Wei5 block,Jiangsu oilfield.The degradation ratio of heavy oil could reach 99.7 mg/d after 7-day aerobic culture in 54 ℃.Under the same conditions,W3 could perfectlyemulsify the same volume of heavy oil,the viscosity of heavy oil reduced by 76.88％,the content of Saturated hydrocarbon,aromatic hydrocarbon and resins decreased by 4.14％,6.77％and4.33％.When this oil was degraded by the combination of W3 and Geobacillus sp.W12(VW3:VW12=1:1),the two strains not only enhance heavy oil degradation(accelerate the degradation ratio,petroleum component continue to decrease),but also to improve the oil emulsion stability(the average partial size of oil reduced by 56.7％).The viscosity of heavy oil was 20.59 MPa·s,only 3.9％of the original.The experiments of the two strains achieved dual effects of viscosity break by means of heavy oil biodegradation and biological emulsion,which can significantly improve the mobilityof heavy oil.
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