鲑鱼降钙素温度敏感型原位凝胶的制备与评价

摘要

Orthogonal design was used to optimize the prescription of the thermosensitive in situ gel of salmon calcitonin with Chitosan and disodium Glucose phosphate(GP)as the matrix.The drug release behavior with different drug concentration was studied and the fetal rat fibroblast cells was used as model cells to evaluate the biocompatibility.The results showed that the optimized preparation was 12 mg/mL chitosan with viscosity 200~400 mPa·s and 100 mg/mL GP.The gelation temperature was (35.3±0.2)℃,the gelation time was 13.7 ± 0.6 min and the accumulative release percent after 6 h showed that each prescription release less than 50% ultimately within 6 h.The biocompatibility study showed that the extracts of hydrogels had no cytotoxicity.%以壳聚糖和葡萄糖磷酸二钠为基质材料构建鲑鱼降钙素温度敏感型原位凝胶系统,采用正交设计综合评分法优化原位凝胶的处方,考察不同载药量处方的体外释放情况,用胎鼠成纤维细胞进行生物相容性研究.结果表明:优化处方为ρ(壳聚糖)=12 mg/mL,黏度为200~400 mPa·s,ρ(葡萄糖磷酸二钠)=100 mg/mL,所制凝胶水溶液的胶凝温度为(35.3±0.2)℃,胶凝时间为13.7±0.6 min,不同载药量处方体外释药结果显示,在6h的释放时间内,各处方都只有不到50%的药物从凝胶中释放,缓释效果明显;生物相容性研究结果显示,凝胶浸提液在所测定的浓度范围内基本没有细胞毒性.