UV-Vis法测定灵丹草油中总萜类含量

摘要

目的 建立测定灵丹草油中总萜类含量的方法.方法 以β-芹子烯为对照,用5%香草醛-冰乙酸溶液、高氯酸显色,在555 nm波长处测定吸收度.结果 β-芹子烯检测浓度在0.0309～0.13095 mg·mL-1范围内与吸收度呈良好线性关系(r=0.9990),平均回收率为99.38%,RSD=1.23%(n=6).结论 本方法简便、准确,可用于灵丹草挥发油的质量控制.%Objective To establish UV-Vis Spectrophtometry method for determination of total triterpenoids in Laggera pterodonta ( DC. ) Benth oil. Methods β- selinene was measured by spectrophotometry with 5％ vanillin-glacial acetic acid solution and perchloric acid as colordeveloping agent and detected at 555 nm wavelegth. Results The results showed that it had a good linearity ( r=0.999 0 ) between 0.030 9～0.1309 5 mg·mL-1,the average recovery was 99.38％, with RSD of 1.26％. Conclusion The method was simple, accurate and would be used for the quality control of Laggera pterodonta ( DC. ) Benth Oil.