蒙药玉簪花中支托皂苷元及其三个皂苷的体外细胞毒活性研究

摘要

Objective To study the cytotoxicity of 4 compounds isolated from Mongolia herbal medicine Flos Hostae in vitro. Methods Inhibition of compounds 1～4 on MDCK, K562, YAC-1、SMMC-7721 cell are studied by MTT in vitro. Results These four compounds from Flos Hostae showed inhibition on four cell. Compounds 1 and 2 show the toxic effect on MDCK cells, the IC50is 287.38 μg/ml and 5.12 μg/ml, respectively. Compounds 1 inhibited proliferation of the tumor cell K562, YAC-1 and SMMC-7721, IC50are 0.17 μg/ml, 5.38 μ g/ml, 2.84 μg/ml, respectively. Compounds 2 has obvious antiproliferative effects on tumor cell K562, YAC-1 and SMMC-7721. Conclusion Compounds 1 can selectively inhibit the proliferation of these tumor cell lines. Compounds 2 has strong proliferation inhibitory effect on tumor cell lines, but has highly toxicity to normal cells. Compounds 1 and 2 can be used as Quality-Marker of Flos Hostae.%目的 对玉簪花中分离得到的4个单体化合物进行体外细胞毒作用研究.方法 采用MTT实验考察四个化合物对狗肾细胞MDCK的细胞毒性和对人慢性髓源性白血病细胞K562、小鼠淋巴瘤 细胞YAC-1、人肝癌细胞SMMC-7721的体外增殖抑制作用.结果 玉簪花中四个单体化合物对四个细胞株均表现出不同程度的生长抑制作用.化合物1和化合物2对MDCK细胞株的IC50值分别为 287.38μg/ml, 5.12μg/ml.化合物1对三个肿瘤细胞株K562、YAC-1、SMMC-7721的IC50值分别为0.17μg/ml、5.38μg/ml、2.84μg/ml,化合物2对K562、YAC-1、SMMC-7721亦呈现明显的增殖抑制作用.结论 化合 物1对癌细胞具有良好的选择性,化合物2对肿瘤细胞具有较强的增殖抑制作用,但对正常细胞毒性很强.化合物1和2可作为玉簪花药材的质量标志物.