自然流产患者绒毛组织PAI-14G/5G多态性和uPA基因的表达及其意义

摘要

目的:探讨纤溶酶原激活物抑制剂-1(PAI-1)4G/5G多态性和纤溶酶原激活剂(uPA)与自然流产的关系及其在妊娠中的作用.方法:正常早孕(对照组)人工流产和自然流产(实验组)稽留流产的绒毛组织各30例,应用免疫组织化学PV法检测绒毛组织中PAI-1和uPA蛋白表达情况,等位基因特异性-PCR(AS-PCR)法检测PAI-14G/5G多态性的表达情况,RT-PCR法检测uPA mRNA表达情况.结果:① PAI-1蛋白主要在绒毛组织的滋养细胞质中表达,其表达对照组低于实验组(P<0.05).uPA与PAI-1蛋白在细胞中分布一致,其表达水平对照组高于实验组(P<0.05).②PAI-14G/4G基因型和4G等位基因表达水平对照组低于实验组(P<0.05),uPA mRNA表达水平对照组高于实验组(P<0.05).③PAI-1和uPA蛋白在自然流产患者绒毛组织中的表达呈负相关(r=-0.639).结论:PAI-14G/5G多态性和uPA异常表达可能与自然流产的发生有关.%Objective: To explore the correlation of PAI-14G/5G polymorphism and uPA with spontaneous abortion and their effect during pregnancy. Methods: Each of 30 samples was from the villus of normal pregnancy (control group) and spontaneous abortion (experimental group), respectively. Immunchistochchemistry analysis was used to detect the protein expression of PAI-1 and uPA. AS-PCR analysis was used to detect PAI-1 4G/5G polymorphism expression and RT-PCR analysis was used to detect uPA mRNA expression. Results: 1) The expression of PAI-1 protein in spontaneous abortion villus was higher than that of normal pregnancy (P＜ 0.05)and it was mainly expressed in the trophocyte cytoplasm. The expression of uPA protein in spontaneous abortion villus was lower than that of normal pregnancy (P＜0.05) and it's distribution was consistent with the PAI-1.2)The frequencies of 4G/4G genotype and 4G alleles of PAI- 1 were signicantly higher in abortions compared with the control. The expression of uPA mRNA was significantly lower in abortions compared with the control. 3)The correlation of PAI-1 and uPA protein level in spontaneous abortion villus was negative (r=-0.639).Conclusion: PAI-1 4G/5G polymorphism and uPA abnormalities may be related to occurrence of spontaneous abortion.