基质细胞衍生因子-1(SDF-1)表达与复发性流产的关系

摘要

Objective:To investigate the relationship between the expression of stromal cell-derived factor 1 (SDF-1) in serum or trophoblasts and recurrent spontaneous abortions (RSA).Methods:Samples of serum and villus tissues (n=36) were obtained from women undergoing RSA.Serum and villus tissue samples as the control (n=36) were collected from normal pregnant patients who accepted induced abortion in 7 9 weeks of pregnancy.The expressions of SDF-1 protein and mRNA in serum and trophoblasts were determined by enzyme linked immunosorbent assay (ELISA) and real-time reverse transcription polymerase chain reaction (Real-time RT-PCR).Results:SDF-1 protein and mRNA in serum and trophoblasts collected from recurrent spontaneous abortions patients had lower levels than those in normal pregnant patients' serum and trophoblasts (P＜0.01).Conclusion:Lower expressions of SDF-1 protein and mRNA in serum and trophoblasts would be associated with recurrent spontaneous abortions.%目的:探讨复发性流产(recurrent spontaneous abortions,RSA)患者血清及滋养细胞中基质细胞衍生因子-1 (stromal cell-derived factor 1,SDF-1)表达与流产的关系.方法:应用酶联免疫吸附试验及荧光定量聚合酶链反应方法检测RSA患者血清及绒毛组织中SDF-1 mRNA的表达(n=36),同期选取因非意愿妊娠7～9周行人工流产患者的血清及绒毛(n=36)作为对照组.结果:RSA患者血清及滋养细胞中SDF-1蛋白及mRNA的表达均显著低于正常妊娠者,差异有统计学意义(P＜0.01).结论:RSA患者SDF-1蛋白及mRNA低表达与流产发生关系密切.