从土壤底层腐烂植物根茎中筛选出适应30℃条件下的多株纤维素酶产生菌,择优挑选其中5株,对它们逐级进行温度驯化,最终得到一株能适应40℃的菌株CE5',其Cx酶活可达24.73 U/mL.通过对初始pH值、底物浓度、接种量3个影响因子的二次回归正交组合设计,得出各因素对菌株CE5'Cx酶活的影响顺序:接种量＞初始pH值＞底物浓度;各因素的最优水平取值:初始pH值6.49,底物浓度8.55 g/L,接种量6.66％.%Many cellulose-producing strains were isolated from the decaying plant roots under 30 ℃,5 of them were selected because of growing well,and they weredomestication cultured by temperature step by step.Finally,one strain named as CE5'was obtained which can adapt to 40 ℃.Its Cx enzymatic activity can reached 24.73 U/mL.Through the quadratic regression orthogonal combination designfor three factorssuch as initial pH value,substrateconcentration and inoculationvolume,the sequence of Cx enzymatic activity of CE5'effected by them are as follows:inoculationvolume＞initial pH value＞substrateconcentration.The optimal level value of three factors are as follows:initial pH value is 6.49,substrateconcentration is 8.55 g/L,inoculationvolume is 6.66％.
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