维生素E对炔雌醚致雄性小鼠生殖毒性的保护作用

摘要

采用细胞化学和酶联免疫吸附试验（ELISA）探讨维生素 E 对炔雌醚致小鼠生殖毒性的保护作用。将15只8周龄雄性昆明小鼠随机分为3组，适应饲养1周后分别灌胃橄榄油、橄榄油＋炔雌醚、橄榄油＋炔雌醚＋维生素 E，每天1次，连续3周。处理结束后，睾丸、附睾、精囊腺及前列腺称重；取睾丸，测定抗氧化能力；取附睾，检测精子品质。结果显示，1 mg/kg 炔雌醚组显著降低生殖器官的重量，抑制器官发育；降低抗氧化酶活性、升高活性氧含量，破坏抗氧化能力；造成精子品质下降。100 mg/kg维生素 E 组显著升高生殖器官重量、抗氧化酶活性及精子品质。推测增强抗氧化酶活性是维生素 E 对生殖功能保护作用的途径之一，其深层机制有待进一步研究。%This study aimed to investigate the protective effect of vitamin E on quinestrol induced reproduc-tive toxicity in mice by using the cell chemistry and enzyme linked immunosorbent assay (ELISA)method. 15 8-week old male Kunming mice were randomly divided into 3 groups and adaptively feed for 1 week,and then treated by intragastric administration of 50 μL olive oil containing 0.1 mg/kg quinestrol,1 mg/kg quinestrol+100 mg/kg vitamin E body weight respectively,1 times a day,for 2 consecutive weeks.At the end of treatment,testes,epididymes and gonads were separated and weighed;testes were treated for an-tioxidant activity assay;epididymes were treated for sperm quality assay.The results showed that 1 mg/kg quinestrol significantly reduced reproductive organ weights,inhibited organ development;reduced the ac-tivity of antioxidant enzymes,increased content of active oxygen damage;declined the sperm quality.100 mg/kg vitamin E significantly increased the weight of reproductive organs,antioxidase activity and sperm quality.The results indicated that increasing antioxidase activity is one of the ways of the protective effects of vitamin E on reproductive function,and the underlying mechanism remains to be further studied.