Three strains of Escherichia coli were isolated from eggs in order to investigate the bacterial infection status of eggs in Kunming,and provide the practical reference for egg production.In addition,the associated features of isolated strains were studied,including virulence genes,resistance genes,animal pathogenicity and drug sensitivity test.The results showed that the virulence gene carrying rate of 75% and the resistance gene rate of 77.3% were found in the the strain KM1;the virulence gene rate of 91.7% and the resistance genes rate of 50% were found in the the strain KM3;while,the rate of the KM2 mediated between the KM1 and KM3.The strain KM1 is non-pathogenic,the KM2 is low-pathogenic,and the KM3 is moderately pathogenic.Drug sensitivity test showed that the isolates were sensitive to cefotaxime and ceftriaxone sodium,however,resistant to gentamicin and ampicillin at different levels.The inhibitory effects of Chinese herbal medicine on 3 strains were different.In comparison,the Forsythia suspensa and pine needles had better anti-bacterial effects.The results provided theoretical basis for further investigation and control of Escherichia coli in eggs.%为了调查鸡蛋中大肠埃希菌的感染情况,为生产实践提供参考,从昆明市区鸡蛋中分离出了3株大肠埃希菌,并对其毒力基因、耐药基因、动物致病性及药物敏感性进行了研究.结果表明,菌株KM1携带的毒力基因检出率75%,耐药基因检出率77.3%;菌株KM2携带的毒力基因和耐药基因检出率居中;菌株KM3毒力基因检出率91.7%,耐药性基因检出率50%.动物毒力检测结果KM1为不致病菌株,KM2为低致病菌株,KM3为中度致病菌株.药敏试验结果显示,分离菌株对头孢噻肟、头孢曲松钠敏感,对庆大霉素、氨苄西林等具有不同程度的耐药性.中草药对3株大肠埃希菌的抑制效果各有所不同,相比之下连翘与松针的效果较好.本试验为研究昆明市区鸡蛋中大肠埃希菌污染与防控提供了理论依据.
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