猪源大肠埃希菌药敏试验及耐药基因检测

摘要

In order to investigate the drug resistance of aminoglycosides and the prevalence of aadA1 and aph (3′)resistance genes in Escherichia coli of a large-scale pig farm in Yunnan province,the KB (Kirby-Bau-er)method was used to detect the drug resistance of 51 E .coli isolates to 6 aminoglycosides and the aph (3′)and aadA1 genes were amplified by PCR.The results showed that the resistant rates of streptomycin, neomycin,tobramycin,gentamicin,amikacin and kanamycin were 49.0%,35.3%,47.1%,43.1%,15.7%and 70.6%,respectively.The positive detection rates of aminoglycosides resistance genes were aph (3′) 86.27% and aadA1 54.90%.The drug resistance of E.coli was widespread in the pig farm,and there were multiple drug resistances to aminoglycoside antibiotics.Among them,the resistance rate of two resistant genes was high.Therefore,the pig farm should be targeted to use drugs,regulate the use of antibiotics, thereby delay and reduce the emergence of drug resistance,and promote the development of social public health.%为调查云南省某规模化猪场大肠埃希菌(E .coli )对氨基糖苷类药物的耐药性及耐药基因 aa-dA1 和aph(3′)的携带情况,采用K-B(Kirby-Bauer)法检测 51 株E .coli对 6 种氨基糖苷类抗生素的耐药性,同时对2 个目的基因片段 aph(3′)和 aadA1 进行 PCR扩增.结果显示,51 株猪E .coli对链霉素、新霉素、妥布霉素、庆大霉素、阿米卡星、卡那霉素的耐药率分别为 49.0%、35.3%、47.1%、43.1%、15.7%及70.6%;利用PCR技术检测氨基糖苷类药物的相关耐药基因,其阳性检出率依次为aph(3′)(86.27%)和aa-dA1(54.90%).结果表明,云南楚雄某猪场撒坝猪E.coli耐药性普遍存在,对氨基糖苷类抗生素存在多重耐药,其中以二重耐药为主,2 个耐药基因检出率偏高.因此,猪场应该针对性选择用药,规范使用抗生素,从而延缓和减少耐药性的产生,促进社会公共卫生的发展.