超临界流体强化溶液分散法制备Eudragit S100纳米颗粒

摘要

目的:制备EudragitS100纳米颗粒.方法:采用超临界流体强化溶液分散(SEDS)法制备,考察了EudragitS100浓度、超临界CO2流速、溶液流速、压力、温度对EudragitS100纳米粒形貌和粒径的影响,并用场发射扫描式电子显微镜、激光粒度分析仪、差示扫描量热仪、傅里叶变换红外光谱仪对样品进行表征.结果:SEDS法可以制备球形的、粒径分布窄的EudragitS100纳米粒,所得纳米粒的平均粒径在90～220nm之间.降低EudragitS100浓度和温度、升高压力有利于制备形貌好、粒径小的纳米粒；提高超临界CO2流速和降低溶液流速也有利于制备粒径小的纳米粒,但当超临界CO2流速升高至4kg/h或溶液流速降低至0.5 ml/min时,纳米粒的产率较低.SEDS处理后EudratitS100仍以无定形态存在,且SEDS过程没有对EudratitS100的化学键造成破坏.结论:采用SEDS法可用于EudragitS100纳米粒的制备,工艺简单可行.%Objective:To explore the relationship between job types (brain workers versus manual workers) and metabolic related phenotypes. Methods: 127 participants (30-75 years) were randomly selected from Taizhou city. The participants received a physical examination by trained investigators, including measurement of height, weight, waist and hip circumferences and blood pressure. In addition, we measured the level of 4 lipid metabolism related markers (LDLR, MTTP, Apo A5, Apo B100) using Elisa. The relationship between job types (brain workers versus manual workers) and metabolic related phenotypes were tested by T test and x2 test. Results: There was statistical difference on energy expenditure and BMI between two groups (P