新型磁性纳米脂质复合物对肝癌细胞和肝细胞的作用研究

摘要

Objective: To investigate the effect of new magnetic nanoliposomes for hepatoma carcinoma cell and hepatocyte. Methods: Hepatoma carcinoma cell (Hep-G2) and hepatocyte (L-02) were respectively mixed with new magnetic nanoliposomes of different concentration. Hepatoma carcinoma cell(Hep-G2) and hepatocyte(L-02) were devided into several groups :blank control group, different iron concentration nanoliposome groups, without iron concentration nanoliposome group. Cell toxicity was detected by methyl thiazolyl tetrazolium(MTT) method, screening suitable concentration and time. Cell phagocytosis was detected by Prussian blue stain and electron microscope. Cell iron content was detected by atomic absorption spectrophotometer. The developing effect of new magnetic nanoliposomes in vitro was detected by magnetic resonance imaging (MRI). Results: Iron content of new magnetic nanoliposomes above 0.238 n,g/ml, 24 hours cell toxicity was obviously. Inhibition ratio of hepatoma carcinoma cell (Hep-G2) and hepatocyte (L-02) were surpassing 40%. The suitable concentration was below 0.238μg/ml.The highest positive rate of 24 hours Prussian blue stain respectively were 5.5% and 1.25%. 24 hours the highest cell iron content respectively were 0.675pg/cell and 0.460pg/cell. There was no particle material in blank control group and without iron concentration nanoliposome group. There was a little particle material different iron concentration nanoliposome groups. There was no signal expression of hepatoma carcinoma cell (Hep-G2) and hepatocyte (L-02) in 24 hours MRI interesting area. Conclusion: Iron concentration of new magnetic nanoliposomes below 0.238μg/ml, 24 hours cell toxicity was low; The rate of hepatoma carcinoma cell phagocytosis was higher than hepatocyte; Inside cell iron content of new magnetic nanoliposomes was low, and the developing effect in vitro with magnetic resonance imaging was not good.%目的:研究新型磁性纳米脂质复合物对肝癌细胞和肝细胞的作用.方法:将肝癌细胞(Hep-G2)和肝细胞(L-02)根据加入的不同浓度新型纳米脂质复合物,各自分为空白对照组、不同浓度含铁脂质体组、空脂质体组,用MTT法观察各组细胞毒性,筛选出适合的浓度和时间；用普鲁士蓝染色、电镜检测观察各组细胞吞噬情况；用原子分光光度计观察各组细胞内铁含量；用细胞磁共振观察新型纳米脂质复合物体外显影效果.结果:新型磁性纳米脂质复合物含铁浓度在0.238μg/ml以上时,24小时有较强的细胞毒性,对肝癌细胞和肝细胞的抑制率均超过40％,故以下实验选择含铁浓度在0.238μg/ml以下；肝癌细胞和肝细胞与各浓度新型磁性纳米脂质复合物共同培养,24小时普鲁士蓝染色最高阳性率分别为5.5％和1.25％,24小时细胞内铁含量最高值分别为0.675pg/cell和0.460pg/cell; 24小时电镜观察肝癌细胞和肝细胞空白对照组均未见颗粒样物质,各含铁脂质体组和空脂质体组均可见颗粒样物质；24小时肝癌细胞和肝细胞磁共振感兴趣区均未见明显信号表达.结论:新型磁性纳米脂质复合物含铁浓度在0.238μg/ml以下时,24小时细胞毒性较小；肝癌细胞对新型磁性纳米脂质复合物的吞噬作用稍高于肝细胞对其的吞噬；进入细胞内的新型磁性纳米脂质复合物含铁量低,体外显影效果不佳.