Objective:The biological effects of low intensity laser have gained extensive interests from both the research scientists and clinical doctors.Numerous evidences have proven that low intensity laser was capable of promoting the soft tissue repair.However,the exact mediating effects of low intensity laser on diabetic wound healing is still poorly understood.In this study,we aimed to systematically evaluate the therapeutic effects of low intensity laser with 650 nm wavelength on the diabetes-induced epidermal wound healing in rats.Methods:Three-month old male Sprague-Dawley (SD) rats were given a single intravenous administration of streptozotocin (STZ) at a dose of 50 mg/kg versus body weight.Mice with fasting blood glucose concentration higher than 16.7 mmol/L were used in the experiment.One open circular wound vith 2-cm diameter in the dorsum was created on either normal or diabetic mice.Sixteen normal mice were used as the Control group,and 32 diabetic mice were randomly divided into Diabetes or Laser treatment group.The rats in Laser treatment group were exposed at 650-um low intensity laser for 2 h per day.On Day 5,12 and 19,four rats in each group were euthanized,and the gross wound area and tensile strength of the soft tissue were respectively qualified and compared.Results:Compared with the rats in Control group,diabetes significantly delayed the wound healing processes and decreased the tissue tensile strength at Day 5,12 and 19 (P＜0.05).Diabetes also increased the gross time for wound healing (P＜0.05).However,the delayed processes for diabetic wound healing were obviously reversed by the laser treatment,evidenced by significantly accelerated process in the epidermal wound healing and increased tissue tensile strength at Day 5,12 and 19,as well as dramatically reduced wound healing time (P＜0.05).Conclusion:Low intensity laser with 650 nm is able to remarkably accelerate the diabetic wound healing process in rats.The wound repair process in diabetic rats after laser stimulation is close to that in normal rats.Our findings indicate that the laser stimulation at the wavelength of 650 nm possesses great potential to be widely used in clinics for the treatment of wound healing in diabetic patients.%目的:低强度激光的生物效应已经得到广大科研工作者和临床医生的广泛关注,诸多研究证明低强度激光能够促进创伤组织的修复,但是其对于糖尿病创伤的组织愈合效果目前仍鲜有报道.本文旨在系统探索650 nm波长的低强度激光照射对糖尿病大鼠皮肤创伤愈合的作用效果.方法:3月龄雄性SD大鼠,腹腔注射50 mg/kg的链脲做菌素,3天后检测空腹血糖值,高于16.7mmol/L的大鼠用于实验.于48只大鼠(32只糖尿病+16只正常)的背部构建直径2 cm的圆形创伤切口,将大鼠随机分为对照组、糖尿病组、糖尿病激光治疗组,每组16只,激光组大鼠伤口每天暴露于650 nm激光下2h.每组分别于第5、12、19天处死4只大鼠,对其进行创伤愈合率和创伤表皮组织生物力学实验评估.结果:相比于对照组,糖尿病大鼠的伤口愈合速度在第5、12、19天均显著降低(P＜0.05),伤口完全愈合时间显著增加(P＜0.05),伤口抗张强度显著下降(P＜0.05).低强度激光刺激显著提高了第5、12、19天大鼠伤口的愈合速度(P＜0.05),显著降低了伤口的愈合时间(P＜0.05),显著提高了伤口抗张强度(P＜0.05).结论:650nm低强度激光能够显著加速糖尿病大鼠创伤的延迟愈合,使其愈合速度接近于正常状态的组织,该研究揭示了650nm波长下的低强度激光治疗具有良好的用于促进糖尿病患者伤口愈合临床应用前景.
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