目的 了解员工暴露前接种人用狂犬病疫苗后的免疫效果,并对比使用两种不同试剂盒检测抗体阳转率是否存在差异.方法 采集员工接种人用狂犬病疫苗( 地鼠肾细胞) 以及冻干人用狂犬病疫苗( Vero细胞) 共计172例,分别使用两种狂犬病毒抗体检测试剂盒( 试剂盒A、B) 进行检测,统计血清中狂犬病毒IgG抗体的水平,计算阳转率并比较差异.结果使用试剂盒A检测接种人用狂犬病疫苗( 地鼠肾细胞) 者血清样本的阳转率为91.7％,接种冻干人用狂犬病疫苗( Vero细胞) 者血清样本的阳转率为51.0％;使用试剂盒B检测接种人用狂犬病疫苗( 地鼠肾细胞) 者血清样本的阳转率为100.0％,接种冻干人用狂犬病疫苗( Vero细胞) 者血清样本的阳转率为74.5％.使用试剂盒B检测接种人用狂犬病疫苗( 地鼠肾细胞) 者血清样本的阳转率比试剂盒A高8.3％,使用试剂盒B检测接种冻干人用狂犬病疫苗( Vero细胞) 者血清样本的阳转率比试剂盒A高23.5％.结论两种不同试剂盒上检测的抗体阳转率都反映出人用狂犬病疫苗( 地鼠肾细胞) 比冻干人用狂犬病疫苗( Vero细胞) 的免疫效果好;使用两种不同试剂盒检测抗体阳转率的差异均具有统计学意义.%Objective To understand the effect of immunization with rabies vaccines for human use before exposure and to compare the difference in seroconversion rate for antibody against rabies virus between 2 different detection kits. Methods A total of 172 staff members were inoculated PHK cell ( hamster-kidney-cell) rabies vaccines and Vero-cell rabies vaccines, respectively, which were detected IgG antibody level in serum by 2 kinds of antibody detection kits ( A and B) against rabies virus, and seroconversion rate were evaluated and the difference compared. Results The detected seroconversion rate was 91.7％ for staff members inoculated PHK cell rabies vaccine, the same rate was 51.0％ for staff members inoculated Vero-cell rabies vaccine by both using kit A. The detected seroconversion rate was 100％ for staff members inoculated PHK cell rabies vaccine, the same rate was 74.5％ for staff members inoculated Vero-cell rabies vaccine by both using kit B.The seroconversion rate was 8.3％ higher by using kit B than kit A for staff members inoculated PHK cell rabies vaccine, the seroconversion rate was 23.5％ higher by using kit B than kit A for staff members inoculated Vero-cell rabies vaccine. Conclusion The detected seroconversion rates showed that PHK rabies vaccine was better than the Vero-cell rabies vaccine. There were significant differences in detection of antibody seroconversion rate by both of detection kits.
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