原子力显微镜在纳米生物材料研究中的应用

摘要

In the past two decades, atomic force microscopy ( AFM) has been widely used for studying nanobiomaterials related research. AFM is a powerful tool that can reveal the surface structure and mechanical properties of nanobiomaterials, and is also known as a nanofabrication tool to manipulate and process nanobiomaterials. The focus of this review is on the recent progress in the applications of AFM for nanobiomaterials, which mainly includes imaging, force measurements, and nanofabrication of nanobiomaterials. AFM can be used to image the topography of nanobiomaterials and analyze the feature height and surface roughness, image the dynamic process of nanobiomaterials related processes in situ. AFM phase imaging can be used to image some surface features of nanobiomaterials those AFM height imaging cannot detect. AFM force curves can be applied to measure the adhesion force between tip and nanobiomaterials, and measure the intermolecular and intramolecular forces of nanobiomaterials. AFM nanoindentation can be applied to measure the mechanical properties (elasticity, Young's modulus, hardness, nanofracture behavior, etc) of nanobiomaterials. In addition, AFM has been explored to fabricate nanobiomaterials in a precise, controllable and reproducible fashion. In summary, as a prowerful nanotechnological tool, AFM has provided an ideal surface-analysis and surface-fabrication tool in the nanobiomaterials related research.%在过去的20年里,原子力显微镜(AFM)在纳米生物材料领域有着广泛的应用.AFM可有力地揭示纳米生物材料的表面结构与力学性质,并且可作为纳米加工工具对其进行操作与处理.本文综述了AFM在纳米生物材料中的最新应用进展,包括纳米生物材料的成像与表征,力学性能测量和纳米加工.AFM可用来观察纳米生物材料的表面形貌并对其特征高度和表面粗糙度进行分析,还可对其动态过程进行原位观察.通过AFM相图还可得到有时候高度图无法获取的一些表面特征.AFM力曲线可用于测量针尖与纳米生物材料之间的黏附力及分子内外的相互作用力.AFM纳米压痕技术可用来测量材料的相关力学性质(弹力,杨氏模量,硬度,纳米断裂行为等).此外,AFM也已经被探索用于精准、可控、可重复地加工纳米生物材料.总之,作为一个强大的纳米技术工具,AFM已成为纳米生物材料相关研究领域的一个理想的表面分析和表面加工工具.